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Injectable osteogenic microtissues containing mesenchymal stromal cells conformally fill and repair critical-size defects.
Annamalai, Ramkumar T; Hong, Xiaowei; Schott, Nicholas G; Tiruchinapally, Gopinath; Levi, Benjamin; Stegemann, Jan P.
Afiliación
  • Annamalai RT; Department of Biomedical Engineering, University of Michigan, Ann Arbor, United States.
  • Hong X; Department of Biomedical Engineering, University of Michigan, Ann Arbor, United States.
  • Schott NG; Department of Biomedical Engineering, University of Michigan, Ann Arbor, United States.
  • Tiruchinapally G; Department of Biomedical Engineering, University of Michigan, Ann Arbor, United States.
  • Levi B; Department of Surgery, University of Michigan, Ann Arbor, United States.
  • Stegemann JP; Department of Biomedical Engineering, University of Michigan, Ann Arbor, United States. Electronic address: jpsteg@umich.edu.
Biomaterials ; 208: 32-44, 2019 07.
Article en En | MEDLINE | ID: mdl-30991216
Repair of complex fractures with bone loss requires a potent, space-filling intervention to promote regeneration of bone. We present a biomaterials-based strategy combining mesenchymal stromal cells (MSC) with a chitosan-collagen matrix to form modular microtissues designed for delivery through a needle to conformally fill cavital defects. Implantation of microtissues into a calvarial defect in the mouse showed that osteogenically pre-differentiated MSC resulted in complete bridging of the cavity, while undifferentiated MSC produced mineralized tissue only in apposition to native bone. Decreasing the implant volume reduced bone regeneration, while increasing the MSC concentration also attenuated bone formation, suggesting that the cell-matrix ratio is important in achieving a robust response. Conformal filling of the defect with microtissues in a carrier gel resulted in complete healing. Taken together, these results show that modular microtissues can be used to augment the differentiated function of MSC and provide an extracellular environment that potentiates bone repair.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteogénesis / Células Madre Mesenquimatosas Límite: Animals Idioma: En Revista: Biomaterials Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteogénesis / Células Madre Mesenquimatosas Límite: Animals Idioma: En Revista: Biomaterials Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos