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Electrochemical Immunosensor for TNFα-Mediated Inflammatory Disease Screening.
Cruz, Andrea; Queirós, Raquel; Abreu, Catarina M; Barata, Catarina; Fernandes, Rosa; Silva, Rufino; Ambrósio, Antonio F; Soares-Dos-Reis, Ricardo; Guimarães, Joana; Sá, Maria José; Relvas, João B; Freitas, Paulo P; Mendes Pinto, Inês.
Afiliación
  • Cruz A; International Iberian Nanotechnology Laboratory , Braga 4715-330 , Portugal.
  • Queirós R; International Iberian Nanotechnology Laboratory , Braga 4715-330 , Portugal.
  • Abreu CM; International Iberian Nanotechnology Laboratory , Braga 4715-330 , Portugal.
  • Barata C; Swansea University Medical School , Swansea SA2 8PP , United Kingdom.
  • Fernandes R; International Iberian Nanotechnology Laboratory , Braga 4715-330 , Portugal.
  • Silva R; Instituto Superior Técnico , University of Lisbon , Lisbon 1649-004 , Portugal.
  • Ambrósio AF; Coimbra Institute for Clinical and Biomedical Research, Faculty of Medicine , University of Coimbra , Coimbra 3004-531 , Portugal.
  • Soares-Dos-Reis R; CNC.IBILI , University of Coimbra , Coimbra 3000-548 , Portugal.
  • Guimarães J; Coimbra Institute for Clinical and Biomedical Research, Faculty of Medicine , University of Coimbra , Coimbra 3004-531 , Portugal.
  • Sá MJ; Coimbra University Hospital , Coimbra 3000-075 , Portugal.
  • Relvas JB; Coimbra Institute for Clinical and Biomedical Research, Faculty of Medicine , University of Coimbra , Coimbra 3004-531 , Portugal.
  • Freitas PP; CNC.IBILI , University of Coimbra , Coimbra 3000-548 , Portugal.
  • Mendes Pinto I; Neurology Department , Centro Hospitalar de São João , Porto 4200-319 , Portugal.
ACS Chem Neurosci ; 10(6): 2676-2682, 2019 06 19.
Article en En | MEDLINE | ID: mdl-30985099
Inflammation associated with cancer, neurodegenerative, ocular, and autoimmune diseases has a considerable impact on public health. Tumor necrosis factor alpha (TNFα) is a key mediator of inflammatory responses, responsible for many of the systemic manifestations during the inflammatory process. Thus, inhibition of TNFα is a commonplace practice in the treatment of these disorders. Successful therapy requires the ability to determine the appropriate dose of anti-TNFα drugs to be administered in a timely manner, based on circulating TNFα levels. In this Letter, we report the development of an immunosensor technology able to quantify TNFα at the picogram level in relevant human body fluids, holding the potential to early detect inflammation  and monitor TNFα levels during treatment, enabling TNFα-targeted treatments to be tailored according to the immune status of an individual patient. This immunosensor technology is significantly more rapid and sensitive than conventional enzyme linked immunosorbent assays, maintaining high specificity and requiring small sample volumes. These features might also be advantageous in the context of personalized medicine, as this analytical platform can deliver advanced diagnostics and reduce clinical burden.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Factor de Necrosis Tumoral alfa / Espectroscopía Dieléctrica Tipo de estudio: Diagnostic_studies / Screening_studies Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: ACS Chem Neurosci Año: 2019 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Factor de Necrosis Tumoral alfa / Espectroscopía Dieléctrica Tipo de estudio: Diagnostic_studies / Screening_studies Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: ACS Chem Neurosci Año: 2019 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Estados Unidos