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Biocompatibility and immunogenic response to recombinant honeybee silk material.
Sutherland, Tara D; Vashi, Aditya V; Kardia, Egi; Sriskantha, Alagacone; Rapson, Trevor D; Hall, Robyn N; Werkmeister, Jerome A.
Afiliación
  • Sutherland TD; CSIRO, Black Mountain, GPO Box 1700, Acton, Canberra, Australian Capital Territory, 2601, Australia.
  • Vashi AV; CSIRO, Black Mountain, GPO Box 1700, Acton, Canberra, Australian Capital Territory, 2601, Australia.
  • Kardia E; CSIRO, Black Mountain, GPO Box 1700, Acton, Canberra, Australian Capital Territory, 2601, Australia.
  • Sriskantha A; CSIRO, Black Mountain, GPO Box 1700, Acton, Canberra, Australian Capital Territory, 2601, Australia.
  • Rapson TD; CSIRO, Black Mountain, GPO Box 1700, Acton, Canberra, Australian Capital Territory, 2601, Australia.
  • Hall RN; CSIRO, Black Mountain, GPO Box 1700, Acton, Canberra, Australian Capital Territory, 2601, Australia.
  • Werkmeister JA; CSIRO, Black Mountain, GPO Box 1700, Acton, Canberra, Australian Capital Territory, 2601, Australia.
J Biomed Mater Res A ; 107(8): 1763-1770, 2019 08.
Article en En | MEDLINE | ID: mdl-30983124
If tolerated in biological environments, recombinant structural proteins offer the advantage that biological cues dictating cell attachment and material degradation can be modified as required for clinical application using genetic engineering. In this study, we investigate the biological response to materials generated from the recombinant honeybee silk protein, AmelF3, a structural protein that can be produced at high levels by fermentation in Escherichia coli. The protein can be readily purified from E. coli host cell proteins after transgenic production and fabricated into various material formats. When implanted subcutaneously according to International Standard ISO 10993 tests, materials generated from the purified recombinant protein were found to be noncytotoxic, inducing a transient weak immunogenic response and a chronic inflammatory response that resolved over time. While preliminary, this study supports the ongoing development of materials generated from this protein for biomedical applications. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1763-1770, 2019.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Abejas / Materiales Biocompatibles / Proteínas Recombinantes / Seda Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Biomed Mater Res A Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2019 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Abejas / Materiales Biocompatibles / Proteínas Recombinantes / Seda Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Biomed Mater Res A Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2019 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos