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Carrying a 112 bp-segment in Helicobacter pylori dupA may associate with increased risk of duodenal ulcer.
Fatahi, Golzar; Talebi Bezmin Abadi, Amin; Peerayeh, Shahin Najar; Forootan, Mojgan.
Afiliación
  • Fatahi G; Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
  • Talebi Bezmin Abadi A; Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Electronic address: Amin.talebi@modares.ac.ir.
  • Peerayeh SN; Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
  • Forootan M; Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Infect Genet Evol ; 73: 21-25, 2019 09.
Article en En | MEDLINE | ID: mdl-30981881
The discovery of Helicobacter pylori in 1983 challenged researchers around the world to identify this pathogen's major virulence factors. The main rationale for this kind of research was to identify a biomarker associated with specific diseases following H. pylori colonization. Among different investigated virulence factors, duodenal ulcer promoting gene A (dupA) has been found to be associated with duodenal ulcer (DU), but its effect was different in various geographical regions. To determine the prevalence of dupA, we applied both classic primer pairs and our newly developed primers producing a highly conserved segment in PCR method. In our survey, 143 (47%) H. pylori isolates were obtained from 304H. pylori-colonized individuals [age range of 19-92; 113 (37%) males with the mean age of 50 and 191 (63%) females with the mean age of 49]. The presence of the dupA gene was investigated by using the different specific primers. The prevalence of the 112 bp segment isolated from H. pylori strains recovered from DU, GU and atrophy groups were significantly higher (81%, p value = .002, 64%, p = .065, 68% and p = .047 38%, respectively) than our control group, where the prevalence of the 112 bp segment was only 38%. Interestingly, a significant relationship was observed between the occurrence of DU and the presence of the 112 bp segment [p = .002; OR: 6.98; (95% CI: 1.94-25.00)]. Taken as a whole, we believe the 112 bp region of H. pylori dupA may serve as the first detected biomarker for the early detection of DU in patients admitted to hospitals.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Helicobacter pylori / Infecciones por Helicobacter / Factores de Virulencia / Susceptibilidad a Enfermedades / Úlcera Duodenal Tipo de estudio: Etiology_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies / Screening_studies Límite: Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Infect Genet Evol Asunto de la revista: BIOLOGIA / DOENCAS TRANSMISSIVEIS / GENETICA Año: 2019 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Helicobacter pylori / Infecciones por Helicobacter / Factores de Virulencia / Susceptibilidad a Enfermedades / Úlcera Duodenal Tipo de estudio: Etiology_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies / Screening_studies Límite: Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Infect Genet Evol Asunto de la revista: BIOLOGIA / DOENCAS TRANSMISSIVEIS / GENETICA Año: 2019 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Países Bajos