Your browser doesn't support javascript.
loading
Efficacy of 5-Aminolevulinic Acid in Photodynamic Detection and Photodynamic Therapy in Veterinary Medicine.
Osaki, Tomohiro; Yokoe, Inoru; Sunden, Yuji; Ota, Urara; Ichikawa, Tomoki; Imazato, Hideo; Ishii, Takuya; Takahashi, Kiwamu; Ishizuka, Masahiro; Tanaka, Tohru; Li, Liming; Yamashita, Masamichi; Murahata, Yusuke; Tsuka, Takeshi; Azuma, Kazuo; Ito, Norihiko; Imagawa, Tomohiro; Okamoto, Yoshiharu.
Afiliación
  • Osaki T; Joint Department of Veterinary Clinical Medicine, Faculty of Agriculture, Tottori University, 4-101 Koyama-Minami, Tottori 680-8553, Japan. tosaki@muses.tottori-u.ac.jp.
  • Yokoe I; Joint Department of Veterinary Clinical Medicine, Faculty of Agriculture, Tottori University, 4-101 Koyama-Minami, Tottori 680-8553, Japan. inoru.y@gmail.com.
  • Sunden Y; Joint Department of Veterinary Clinical Medicine, Faculty of Agriculture, Tottori University, 4-101 Koyama-Minami, Tottori 680-8553, Japan. sunden@tottori-u.ac.jp.
  • Ota U; SBI Pharmaceuticals Co., Ltd., Tokyo 106-6020, Japan. uota@sbigroup.co.jp.
  • Ichikawa T; SBI Pharmaceuticals Co., Ltd., Tokyo 106-6020, Japan. toichika@sbigroup.co.jp.
  • Imazato H; SBI Pharmaceuticals Co., Ltd., Tokyo 106-6020, Japan. himazato@sbigroup.co.jp.
  • Ishii T; SBI Pharmaceuticals Co., Ltd., Tokyo 106-6020, Japan. taishii@sbigroup.co.jp.
  • Takahashi K; SBI Pharmaceuticals Co., Ltd., Tokyo 106-6020, Japan. kiwtakah@sbigroup.co.jp.
  • Ishizuka M; SBI Pharmaceuticals Co., Ltd., Tokyo 106-6020, Japan. mishizuk@sbigroup.co.jp.
  • Tanaka T; SBI Pharmaceuticals Co., Ltd., Tokyo 106-6020, Japan. tortanaka@sbigroup.co.jp.
  • Li L; Department of Bio- and Material Photonics, Chitose Institute of Science and Technology, Chitose 066-0012, Japan. liliming@photon.chitose.ac.jp.
  • Yamashita M; Joint Department of Veterinary Clinical Medicine, Faculty of Agriculture, Tottori University, 4-101 Koyama-Minami, Tottori 680-8553, Japan. yamashita@muses.tottori-u.ac.jp.
  • Murahata Y; Joint Department of Veterinary Clinical Medicine, Faculty of Agriculture, Tottori University, 4-101 Koyama-Minami, Tottori 680-8553, Japan. ymurahata@muses.tottori-u.ac.jp.
  • Tsuka T; Joint Department of Veterinary Clinical Medicine, Faculty of Agriculture, Tottori University, 4-101 Koyama-Minami, Tottori 680-8553, Japan. tsuka@muses.tottori-u.ac.jp.
  • Azuma K; Joint Department of Veterinary Clinical Medicine, Faculty of Agriculture, Tottori University, 4-101 Koyama-Minami, Tottori 680-8553, Japan. kazu-azuma@muses.tottori-u.ac.jp.
  • Ito N; Joint Department of Veterinary Clinical Medicine, Faculty of Agriculture, Tottori University, 4-101 Koyama-Minami, Tottori 680-8553, Japan. taromobile@me.com.
  • Imagawa T; Joint Department of Veterinary Clinical Medicine, Faculty of Agriculture, Tottori University, 4-101 Koyama-Minami, Tottori 680-8553, Japan. imagawat@muses.tottori-u.ac.jp.
  • Okamoto Y; Joint Department of Veterinary Clinical Medicine, Faculty of Agriculture, Tottori University, 4-101 Koyama-Minami, Tottori 680-8553, Japan. yokamoto@muses.tottori-u.ac.jp.
Cancers (Basel) ; 11(4)2019 Apr 07.
Article en En | MEDLINE | ID: mdl-30959982
5-Aminolevulinic acid (5-ALA), a commonly used photosensitizer in photodynamic detection (PDD) and therapy (PDT), is converted in situ to the established photosensitizer protoporphyrin IX (PpIX) via the heme biosynthetic pathway. To extend 5-ALA-PDT application, we evaluated the PpIX fluorescence induced by exogenous 5-ALA in various veterinary tumors and treated canine and feline tumors. 5-ALA-PDD sensitivity and specificity in the whole sample group for dogs and cats combined were 89.5 and 50%, respectively. Notably, some small tumors disappeared upon 5-ALA-PDT. Although single PDT application was not curative, repeated PDT+/-chemotherapy achieved long-term tumor control. We analyzed the relationship between intracellular PpIX concentration and 5-ALA-PDT in vitro cytotoxicity using various primary tumor cells and determined the correlation between intracellular PpIX concentration and 5-ALA transporter and metabolic enzyme mRNA expression levels. 5-ALA-PDT cytotoxicity in vitro correlated with intracellular PpIX concentration in carcinomas. Ferrochelatase mRNA expression levels strongly negatively correlated with PpIX accumulation, representing the first report of a correlation between mRNA expression related to PpIX accumulation and PpIX concentration in canine tumor cells. Our findings suggested that the results of 5-ALA-PDD might be predictive for 5-ALA-PDT therapeutic effects for carcinomas, with 5-ALA-PDT plus chemotherapy potentially increasing the probability of tumor control in veterinary medicine.
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies / Prognostic_studies Idioma: En Revista: Cancers (Basel) Año: 2019 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies / Prognostic_studies Idioma: En Revista: Cancers (Basel) Año: 2019 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Suiza