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Nanoparticle assisted nuclear relaxation-based oligonucleotide detection.
Silva, Rebecca N; Vijayan, Anjaly N; Westbrook, Emily; Yu, Zhao; Zhang, Peng.
Afiliación
  • Silva RN; Department of Chemistry, University of Cincinnati, OH, 45221, USA.
  • Vijayan AN; Department of Chemistry, University of Cincinnati, OH, 45221, USA.
  • Westbrook E; Department of Chemistry, University of Cincinnati, OH, 45221, USA.
  • Yu Z; Department of Chemistry, University of Cincinnati, OH, 45221, USA.
  • Zhang P; Department of Chemistry, University of Cincinnati, OH, 45221, USA. Electronic address: peng.zhang@uc.edu.
Anal Chim Acta ; 1062: 118-123, 2019 Jul 25.
Article en En | MEDLINE | ID: mdl-30947987
We present a proof-of-concept "on-off" detection scheme, which uses gadolinium phthalocyanine (GdTcPc)-grafted silica nanoparticles as paramagnetic centers, capable of modifying the transverse relaxation time (T2) of water protons in solution. A DNA strand (as probe) was conjugated to the GdTcPc to act as a recognition element. In the presence of the target DNA, which was complementary to the probe, an increase in the T2 value was detected, with magnitude proportional to the target DNA concentration. The linear range was observed from 30 to 140 nM, with limit of detection of 15 nM. The developed nuclear relaxation-based detection scheme is shown to be a simple, fast and selective method to detect DNA and could be useful in point-of-care diagnostic applications.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oligonucleótidos / Nanopartículas Tipo de estudio: Diagnostic_studies Idioma: En Revista: Anal Chim Acta Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oligonucleótidos / Nanopartículas Tipo de estudio: Diagnostic_studies Idioma: En Revista: Anal Chim Acta Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos