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Isolation, characterization, and PCR-based molecular identification of a siphoviridae phage infecting Shigella dysenteriae.
Shahin, Khashayar; Bao, Hongduo; Komijani, Majid; Barazandeh, Mohadeseh; Bouzari, Majid; Hedayatkhah, Abolghasem; Zhang, Lili; Zhao, Hang; He, Tao; Pang, Maoda; Wang, Ran.
Afiliación
  • Shahin K; State Key Laboratory Cultivation Base of MOST, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, PR China.
  • Bao H; State Key Laboratory Cultivation Base of MOST, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, PR China.
  • Komijani M; Department of Biology, Faculty of Science, Arak University, Arak, 38156-8-8349, Iran.
  • Barazandeh M; State Key Laboratory Cultivation Base of MOST, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, PR China.
  • Bouzari M; Department of Biology, Faculty of Sciences, University of Isfahan, Hezar Jereeb Street, 81746-73441, Isfahan, Iran.
  • Hedayatkhah A; Department of Marine Microbiology and Biogeochemistry, Royal Netherlands Institute for Sea Research and Utrecht University, Den Burg, the Netherlands; Department of Freshwater and Marine Ecology, IBED, University of Amsterdam, Amsterdam, the Netherlands.
  • Zhang L; State Key Laboratory Cultivation Base of MOST, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, PR China.
  • Zhao H; State Key Laboratory Cultivation Base of MOST, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, PR China.
  • He T; State Key Laboratory Cultivation Base of MOST, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, PR China.
  • Pang M; State Key Laboratory Cultivation Base of MOST, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, PR China.
  • Wang R; State Key Laboratory Cultivation Base of MOST, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, PR China; Jiangsu University, Zhenjiang, Jiangsu, 212013, China. Electronic address: ranwang@jaas.ac.cn.
Microb Pathog ; 131: 175-180, 2019 Jun.
Article en En | MEDLINE | ID: mdl-30946984
BACKGROUND: Shigella dysenteriae is one of the members of Shigella genus which was the main responsible of different Shigellosis outbreaks worldwide. The increasing consumption of antibiotics has led to the emergence and spreading of antibiotic-resistant strains. Therefore, finding new alternatives for infection control is essential, one of which is using bacteriophages. MATERIALS AND METHODS: Lytic bacteriophage against Shigella dysenteriae was isolated from petroleum refinery wastewater. Phage morphological and genetic characteristics were studied using TEM, and sequencing, respectively. In addition, the genome size was estimated, and phage resistance to different temperatures and pH, host range, adsorption rate, and one-step growth were investigated. RESULTS: According to the morphology and genetic results, this phage was named vB-SdyS-ISF003. Sequencing of the PCR products revealed that the vB-SdyS-ISF003 phage belongs to the species T1virus, subfamily Tunavirinae of family Siphoviridae. This was the first detected bacteriophage against S. dysenteriae, which belongs to the family Siphoviridae. In addition, its host range was limited to S. dysenteriae. The genome size was about 62 kb. vB-SdyS-ISF003 phage has a number of desirable characteristics including the limited host range to S. dysenteriae, very short connection time, a relatively wide range of temperature tolerance -20 to 50 °C, pH tolerance of 7-9 without significant reduction in the phage titer. CONCLUSION: vB-SdyS-ISF003 is a novel virulent T1virus phage and has the appropriate potential for being used in bio controlling of S. dysenteriae in different condition.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Shigella dysenteriae / Reacción en Cadena de la Polimerasa / Siphoviridae Tipo de estudio: Diagnostic_studies / Prognostic_studies Idioma: En Revista: Microb Pathog Asunto de la revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Año: 2019 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Shigella dysenteriae / Reacción en Cadena de la Polimerasa / Siphoviridae Tipo de estudio: Diagnostic_studies / Prognostic_studies Idioma: En Revista: Microb Pathog Asunto de la revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Año: 2019 Tipo del documento: Article Pais de publicación: Reino Unido