De Novo Plant Transcriptome Assembly and Annotation Using Illumina RNA-Seq Reads.
Methods Mol Biol
; 1933: 265-275, 2019.
Article
en En
| MEDLINE
| ID: mdl-30945191
The ability to identify and quantify transcribed sequences from a multitude of organisms using high-throughput RNA sequencing has revolutionized our understanding of genetics and plant biology. However, a number of computational tools used in these analyses still require a reference genome sequence, something that is seldom available for non-model organisms. Computational tools employing de Bruijn graphs to reconstruct full-length transcripts from short sequence reads allow for de novo transcriptome assembly. Here we provide detailed methods for generating and annotating de novo transcriptome assembly from plant RNA-seq data.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Análisis de Secuencia de ARN
/
Arabidopsis
/
ARN de Planta
/
Biología Computacional
/
Secuenciación de Nucleótidos de Alto Rendimiento
/
ARN Largo no Codificante
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2019
Tipo del documento:
Article
País de afiliación:
Australia
Pais de publicación:
Estados Unidos