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Development of Whole-Porcine Monoclonal Antibodies with Potent Neutralization Activity against Classical Swine Fever Virus from Single B Cells.
Dong, Haisi; Su, Ang; Lv, Dongmei; Ma, Lerong; Dong, Jianwei; Guo, Nannan; Ren, Linzhu; Jiao, Huping; Pang, Daxin; Ouyan, Hongsheng.
Afiliación
  • Dong H; Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Science , Jilin University , Changchun 130062 , Jilin Province , People's Republic of China.
  • Su A; Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Science , Jilin University , Changchun 130062 , Jilin Province , People's Republic of China.
  • Lv D; Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Science , Jilin University , Changchun 130062 , Jilin Province , People's Republic of China.
  • Ma L; Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Science , Jilin University , Changchun 130062 , Jilin Province , People's Republic of China.
  • Dong J; Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Science , Jilin University , Changchun 130062 , Jilin Province , People's Republic of China.
  • Guo N; Department of Immunohaematology and Blood Transfusion , Leiden University Medical Center (LUMC) , 2311 EZ Leiden , The Netherlands.
  • Ren L; Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Science , Jilin University , Changchun 130062 , Jilin Province , People's Republic of China.
  • Jiao H; Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Science , Jilin University , Changchun 130062 , Jilin Province , People's Republic of China.
  • Pang D; Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Science , Jilin University , Changchun 130062 , Jilin Province , People's Republic of China.
  • Ouyan H; Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Science , Jilin University , Changchun 130062 , Jilin Province , People's Republic of China.
ACS Synth Biol ; 8(5): 989-1000, 2019 05 17.
Article en En | MEDLINE | ID: mdl-30935202
Classical swine fever (CSF) is a highly contagious swine disease that causes devastating economic losses. However, there are few efficacious therapeutic antibodies against the CSF virus (CSFV). Accordingly, we isolated two whole-porcine anti-CSFV neutralizing antibodies (NAbs) directly from single B cells sorted using the conserved linear epitope of the CSFV E2 protein and goat anti-pig IgG. These mAbs, termed HK24 and HK44, can bind to the E2 protein by recognizing sites within the conserved linear epitope of E2. In addition, these two mAbs can detect virus infection with high specificity and possess potent neutralizing activity. HK24 and HK44 protect PK-15 cells from CSFV infections in vitro with potent IC50 values of 9.3 and 0.62 µg/mL, respectively. We anticipate that these antibodies can be used as diagnostic and antiviral agents for CSFV and that the method we describe here will accelerate the production of therapeutic antibodies for other viruses.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos B / Proteínas del Envoltorio Viral / Virus de la Fiebre Porcina Clásica / Anticuerpos Monoclonales / Anticuerpos Antivirales Límite: Animals / Humans Idioma: En Revista: ACS Synth Biol Año: 2019 Tipo del documento: Article Pais de publicación: Estados Unidos
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos B / Proteínas del Envoltorio Viral / Virus de la Fiebre Porcina Clásica / Anticuerpos Monoclonales / Anticuerpos Antivirales Límite: Animals / Humans Idioma: En Revista: ACS Synth Biol Año: 2019 Tipo del documento: Article Pais de publicación: Estados Unidos