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Dummy molecularly imprinted polymer based microplate chemiluminescence sensor for one-step detection of Sudan dyes in egg.
He, Tong; Wang, Geng Nan; Liu, Ju Xiang; Zhao, Wei Li; Huang, Jing Jie; Xu, Ming Xin; Wang, Jian Ping; Liu, Jing.
Afiliación
  • He T; College of Veterinary Medicine, Hebei Agricultural University, Baoding, Hebei 071000, China.
  • Wang GN; College of Veterinary Medicine, Hebei Agricultural University, Baoding, Hebei 071000, China.
  • Liu JX; College of Veterinary Medicine, Hebei Agricultural University, Baoding, Hebei 071000, China.
  • Zhao WL; College of Veterinary Medicine, Hebei Agricultural University, Baoding, Hebei 071000, China.
  • Huang JJ; College of Veterinary Medicine, Hebei Agricultural University, Baoding, Hebei 071000, China.
  • Xu MX; College of Veterinary Medicine, Hebei Agricultural University, Baoding, Hebei 071000, China.
  • Wang JP; College of Veterinary Medicine, Hebei Agricultural University, Baoding, Hebei 071000, China. Electronic address: chinawangjp@hotmail.com.
  • Liu J; College of Veterinary Medicine, Hebei Agricultural University, Baoding, Hebei 071000, China. Electronic address: 444937594@qq.com.
Food Chem ; 288: 347-353, 2019 Aug 01.
Article en En | MEDLINE | ID: mdl-30902303
The objective of this study is to report a molecularly imprinted polymer-based chemiluminescence method for determination of Sudan dyes. A dummy-template molecularly imprinted polymer capable of recognizing seven Sudan dyes was first synthesized and its recognition mechanism was studied by using computation simulation method. The polymer was coated in the wells of conventional microplate to prepare a chemiluminescence sensor and the assay process consisted of only one sample-loading step prior to signal acquisition. The optimized sensor was used to determine the seven dyes in egg yolk and the results were confirmed with a high performance liquid chromatography. Results showed that this sensor achieved ultrahigh sensitivity (1.0-5.0 pg/mL), rapid assay process (10 min) and satisfactory recovery (70.5%-92.2%). Furthermore, the sensor could be reused for 5 times. Therefore, this sensor could be used as a useful tool for screening the residues of Sudan dyes in egg.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polímeros / Colorantes / Yema de Huevo / Impresión Molecular / Mediciones Luminiscentes Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Food Chem Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polímeros / Colorantes / Yema de Huevo / Impresión Molecular / Mediciones Luminiscentes Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Food Chem Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido