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Molecular studies of meningococcal and pneumococcal meningitis patients in Ethiopia.
Mihret, Wude; Sletbakk Brusletto, Berit; Øvstebø, Reidun; Siebke Troseid, Anne-Marie; Norheim, Gunnstein; Merid, Yared; Kassu, Afework; Abebe, Workeabeba; Ayele, Samuel; Silamsaw Asres, Mezgebu; Yamuah, Lawrence; Aseffa, Abraham; Petros, Beyene; Caugant, Dominique A; Brandtzaeg, Petter.
Afiliación
  • Mihret W; 1 Department of Microbial and Cellular Molecular Biology, Addis Ababa University, Ethiopia.
  • Sletbakk Brusletto B; 2 Armauer Hansen Research Institute, Addis Ababa, Ethiopia.
  • Øvstebø R; 3 Department of Clinical Chemistry, Oslo University Hospital, Norway.
  • Siebke Troseid AM; 3 Department of Clinical Chemistry, Oslo University Hospital, Norway.
  • Norheim G; 3 Department of Clinical Chemistry, Oslo University Hospital, Norway.
  • Merid Y; 4 Institute of Public Health, Oslo, Norway.
  • Kassu A; 5 Hawassa University, Ethiopia.
  • Abebe W; 5 Hawassa University, Ethiopia.
  • Ayele S; 6 Addis Ababa University, Ethiopia.
  • Silamsaw Asres M; 2 Armauer Hansen Research Institute, Addis Ababa, Ethiopia.
  • Yamuah L; 7 University of Gondar, Ethiopia.
  • Aseffa A; 2 Armauer Hansen Research Institute, Addis Ababa, Ethiopia.
  • Petros B; 2 Armauer Hansen Research Institute, Addis Ababa, Ethiopia.
  • Caugant DA; 6 Addis Ababa University, Ethiopia.
  • Brandtzaeg P; 4 Institute of Public Health, Oslo, Norway.
Innate Immun ; 25(3): 158-167, 2019 04.
Article en En | MEDLINE | ID: mdl-30894090
Neisseria meningitidis infections in sub-Saharan Africa usually present with distinct symptoms of meningitis but very rarely as fulminant septicemia when reaching hospitals. In Europe, development of persistent meningococcal shock and multiple organ failure occurs in up to 30% of patients and is associated with a bacterial load of >106/ml plasma or serum. We have prospectively studied 27 Ethiopian patients with meningococcal infection as diagnosed and quantified with real-time PCR in the cerebrospinal fluid (CSF) and serum. All presented with symptoms of meningitis and none with fulminant septicemia. The median N. meningitidis copy number (NmDNA) in serum was < 3.5 × 103/ml, never exceeded 1.8 × 105/ml, and was always 10-1000 times higher in CSF than in serum. The levels of LPS in CSF as determined by the limulus amebocyte lysate assay were positively correlated to NmDNA copy number ( r = 0.45, P = 0.030), levels of IL-1 receptor antagonist, ( r = 0.46, P = 0.017), and matrix metallopeptidase-9 (MMP-9; r = 0.009). We also compared the inflammatory profiles of 19 mediators in CSF of the 26 meningococcal patients (2 died and 2 had immediate severe sequelae) with 16 patients with Streptococcus pneumoniae meningitis (3 died and 3 with immediate severe sequelae). Of 19 inflammatory mediators tested, 9 were significantly higher in patients with pneumococcal meningitis and possibly linked to worse outcome.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Streptococcus pneumoniae / Epidemias / Meningitis Meningocócica / Meningitis Neumocócica / Neisseria meningitidis Tipo de estudio: Diagnostic_studies / Observational_studies / Risk_factors_studies Límite: Adolescent / Adult / Aged / Child / Child, preschool / Female / Humans / Infant / Male / Middle aged País/Región como asunto: Africa Idioma: En Revista: Innate Immun Asunto de la revista: ALERGIA E IMUNOLOGIA / BACTERIOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Etiopia Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Streptococcus pneumoniae / Epidemias / Meningitis Meningocócica / Meningitis Neumocócica / Neisseria meningitidis Tipo de estudio: Diagnostic_studies / Observational_studies / Risk_factors_studies Límite: Adolescent / Adult / Aged / Child / Child, preschool / Female / Humans / Infant / Male / Middle aged País/Región como asunto: Africa Idioma: En Revista: Innate Immun Asunto de la revista: ALERGIA E IMUNOLOGIA / BACTERIOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Etiopia Pais de publicación: Estados Unidos