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Electron transport pathways in isolated chromoplasts from Narcissus pseudonarcissus L.
Grabsztunowicz, Magda; Mulo, Paula; Baymann, Frauke; Mutoh, Risa; Kurisu, Genji; Sétif, Pierre; Beyer, Peter; Krieger-Liszkay, Anja.
Afiliación
  • Grabsztunowicz M; Molecular Plant Biology, University of Turku, 20520, Turku, Finland.
  • Mulo P; Molecular Plant Biology, University of Turku, 20520, Turku, Finland.
  • Baymann F; Bioénergétique et Ingénierie des Protéines, UMR 7281, CNRS - Aix-Marseille Université, 31, chemin Joseph Aiguier, 13009, Marseille, France.
  • Mutoh R; Institute for Protein Research, Osaka University, Suita, Osaka, 565-0871, Japan.
  • Kurisu G; Institute for Protein Research, Osaka University, Suita, Osaka, 565-0871, Japan.
  • Sétif P; Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ Paris-Sud, Université Paris-Saclay, 91198, Gif-sur-Yvette cedex, France.
  • Beyer P; Faculty of Biology, University of Freiburg, 79104, Freiburg, Germany.
  • Krieger-Liszkay A; Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ Paris-Sud, Université Paris-Saclay, 91198, Gif-sur-Yvette cedex, France.
Plant J ; 99(2): 245-256, 2019 07.
Article en En | MEDLINE | ID: mdl-30888718
During daffodil flower development, chloroplasts differentiate into photosynthetically inactive chromoplasts having lost functional photosynthetic reaction centers. Chromoplasts exhibit a respiratory activity reducing oxygen to water and generating ATP. Immunoblots revealed the presence of the plastid terminal oxidase (PTOX), the NAD(P)H dehydrogenase (NDH) complex, the cytochrome b6 f complex, ATP synthase and several isoforms of ferredoxin-NADP+ oxidoreductase (FNR), and ferredoxin (Fd). Fluorescence spectroscopy allowed the detection of chlorophyll a in the cytochrome b6 f complex. Here we characterize the electron transport pathway of chromorespiration by using specific inhibitors for the NDH complex, the cytochrome b6 f complex, FNR and redox-inactive Fd in which the iron was replaced by gallium. Our data suggest an electron flow via two separate pathways, both reducing plastoquinone (PQ) and using PTOX as oxidase. The first oxidizes NADPH via FNR, Fd and cytochrome bh of the cytochrome b6 f complex, and does not result in the pumping of protons across the membrane. In the second, electron transport takes place via the NDH complex using both NADH and NADPH as electron donor. FNR and Fd are not involved in this pathway. The NDH complex is responsible for the generation of the proton gradient. We propose a model for chromorespiration that may also be relevant for the understanding of chlororespiration and for the characterization of the electron input from Fd to the cytochrome b6 f complex during cyclic electron transport in chloroplasts.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plastidios / Narcissus / Transporte de Electrón Idioma: En Revista: Plant J Asunto de la revista: BIOLOGIA MOLECULAR / BOTANICA Año: 2019 Tipo del documento: Article País de afiliación: Finlandia Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plastidios / Narcissus / Transporte de Electrón Idioma: En Revista: Plant J Asunto de la revista: BIOLOGIA MOLECULAR / BOTANICA Año: 2019 Tipo del documento: Article País de afiliación: Finlandia Pais de publicación: Reino Unido