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Preparation, characterization and application of rod-like chitin nanocrystal by using p-toluenesulfonic acid/choline chloride deep eutectic solvent as a hydrolytic media.
Cao, Shi-Lin; Gu, Wei-Ming; Ou-Yang, Wei-Dong; Chen, De-Chu; Yang, Bao-Yan; Lai, Lin-Hao; Wu, Yi-Da; Liu, Yu-Jia; Zhu, Jie; Chen, Wan-Juan; Gai, Zuo-Qi; Hou, Xue-Dan; Ma, Yong-Zheng; An, Yan-Xia.
Afiliación
  • Cao SL; School of Food Science and Engineering, Foshan University, Foshan 528000, China. Electronic address: shilin.cao@fosu.edu.cn.
  • Gu WM; School of Food Science and Engineering, Foshan University, Foshan 528000, China.
  • Ou-Yang WD; School of Food Science and Engineering, Foshan University, Foshan 528000, China.
  • Chen DC; School of Food Science and Engineering, Foshan University, Foshan 528000, China.
  • Yang BY; School of Food Science and Engineering, Foshan University, Foshan 528000, China.
  • Lai LH; School of Food Science and Engineering, Foshan University, Foshan 528000, China.
  • Wu YD; School of Food Science and Engineering, Foshan University, Foshan 528000, China.
  • Liu YJ; School of Chemical Engineering and Energy Technology, Dongguan University of Technology, Dongguan 523808, China.
  • Zhu J; School of Chemical Engineering and Energy Technology, Dongguan University of Technology, Dongguan 523808, China. Electronic address: zhujie@dgut.edu.cn.
  • Chen WJ; School of Materials Science and Energy Engineering, Foshan University, Foshan 528000, China.
  • Gai ZQ; School of Life Science and Engineering, Foshan University, Foshan University, Foshan 528000, China.
  • Hou XD; School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China.
  • Ma YZ; School of Marine Science and Technology, Tianjin University, Tianjin 300072, China.
  • An YX; College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China.
Carbohydr Polym ; 213: 304-310, 2019 Jun 01.
Article en En | MEDLINE | ID: mdl-30879673
Chitin nanocrystal (ChiNC) was fabricated based on p-toluenesulfonic acid -choline chloride deep eutectic solvent treatment. The obtained ChiNC was about 12-44 nm in width and 206-399 nm in length. The crystalline structure and the functional groups of ChiNC were maintained during the preparation process. Moreover, porcine pancreas lipase (PPL) was successfully immobilized onto the ChiNC to form the immobilized PPL (PPL@ChiNC). The resulting PPL@ChiNC has enzyme loading and activity recovery of 35.6 mg/g and 82.5%, respectively. The thermal stability, pH and temperature adaptabilities of PPL@ChiNC was improved, comparing with free PPL. The demonstrated DES treatment process was efficient for ChiNC preparation and the as-prepared ChiNC exhibited great potentials in biocatalysis and biomedical field.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bencenosulfonatos / Quitina / Colina / Nanopartículas Idioma: En Revista: Carbohydr Polym Año: 2019 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bencenosulfonatos / Quitina / Colina / Nanopartículas Idioma: En Revista: Carbohydr Polym Año: 2019 Tipo del documento: Article Pais de publicación: Reino Unido