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X-ray scanning microscopies of microcalcifications in abdominal aortic and popliteal artery aneurysms.
Giannini, C; Ladisa, M; Lutz-Bueno, V; Terzi, A; Ramella, M; Fusaro, L; Altamura, D; Siliqi, D; Sibillano, T; Diaz, A; Boccafoschi, F; Bunk, O.
Afiliación
  • Giannini C; Institute of Crystallography, National Research Council, via Amendola 122/O, Bari, Bari 70125, Italy.
  • Ladisa M; Institute of Crystallography, National Research Council, via Amendola 122/O, Bari, Bari 70125, Italy.
  • Lutz-Bueno V; Paul Scherrer Institut, Forschungsstrasse 111, Villigen PSI, 5232, Switzerland.
  • Terzi A; Institute of Crystallography, National Research Council, via Amendola 122/O, Bari, Bari 70125, Italy.
  • Ramella M; Department of Health Sciences, University of Piemonte Orientale, Via Solaroli 17, Novara, 28100, Italy.
  • Fusaro L; Department of Health Sciences, University of Piemonte Orientale, Via Solaroli 17, Novara, 28100, Italy.
  • Altamura D; Institute of Crystallography, National Research Council, via Amendola 122/O, Bari, Bari 70125, Italy.
  • Siliqi D; Institute of Crystallography, National Research Council, via Amendola 122/O, Bari, Bari 70125, Italy.
  • Sibillano T; Institute of Crystallography, National Research Council, via Amendola 122/O, Bari, Bari 70125, Italy.
  • Diaz A; Paul Scherrer Institut, Forschungsstrasse 111, Villigen PSI, 5232, Switzerland.
  • Boccafoschi F; Institute of Crystallography, National Research Council, via Amendola 122/O, Bari, Bari 70125, Italy.
  • Bunk O; Department of Health Sciences, University of Piemonte Orientale, Via Solaroli 17, Novara, 28100, Italy.
IUCrJ ; 6(Pt 2): 267-276, 2019 Mar 01.
Article en En | MEDLINE | ID: mdl-30867924
Abdominal aortic and popliteal artery aneurysms are vascular diseases which show massive degeneration, weakening of the vascular wall and loss of the vascular tissue functionality. They are driven by inflammatory, hemodynamical factors and biological alterations that may lead, in the case of an abdominal aortic aneurysm, to sudden and dangerous ruptures of the arteries. Here, human aortic and popliteal aneurysm tissues were obtained during surgical repair, and studied by synchrotron radiation X-ray scanning microdiffraction and small-angle scattering, to investigate the microcalcifications present in the tissues. Data collected during the experiments were transformed into quantitative microscopy images through the combination of statistical approaches and crystallographic methods. As a result of this multi-step analysis, microcalcifications, which are markers of the pathology, were classified in terms of chemical and structural content. This analysis helped to identify the presence of nanocrystalline hy-droxy-apatite and microcrystalline cholesterol, embedded in myofilament, and elastin-containing tissue with low collagen content in predominantly nanocrystalline areas. The generality of the approach allows it to be transferred to other types of tissue and other pathologies affected by microcalcifications, such as thyroid carcinoma, breast cancer, testicular microli-thia-sis or glioblastoma.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: IUCrJ Año: 2019 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: IUCrJ Año: 2019 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido