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A signal amplification system on a lateral flow immunoassay detecting for hepatitis e-antigen in human blood samples.
Si, Jinhong; Li, Jinfeng; Zhang, Ling; Zhang, Weiyun; Yao, Jinxiu; Li, Tingting; Wang, Wenjing; Zhu, Weihang; Allain, Jean-Pierre; Fu, Yongshui; Li, Chengyao.
Afiliación
  • Si J; Department of Transfusion Medicine, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China.
  • Li J; Department of Transfusion Medicine, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China.
  • Zhang L; Shenzhen Key Laboratory of Molecular Epidemiology, Center for Disease Control and Prevention (CDC), Shenzhen, China.
  • Zhang W; Department of Transfusion Medicine, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China.
  • Yao J; Laboratory Department, Military General Hospital of Guangzhou, Guangzhou, China.
  • Li T; Laboratory Department, Yangjiang People's Hospital, Yangjiang, Guangdong, China.
  • Wang W; Department of Transfusion Medicine, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China.
  • Zhu W; Department of Transfusion Medicine, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China.
  • Allain JP; Department of Blood Donation Screening, Shenzhen Blood Center, Shenzhen, China.
  • Fu Y; Department of Transfusion Medicine, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China.
  • Li C; Emeritus Professor, University of Cambridge, Cambridge, UK.
J Med Virol ; 91(7): 1301-1306, 2019 07.
Article en En | MEDLINE | ID: mdl-30851129
Hepatitis B e-antigen (HBeAg) is the secretory form of the nucleocapsid of the hepatitis B virus (HBV), which is a marker of viral replication. In this study, a novel signal amplification system (SAS) based on the lateral flow immunoassay (LFIA) was used for rapid detection of HBeAg in blood samples from patients or blood donors. In this assay, the detection antibody was conjugated with gold nanoparticles (GNPs), and the capture antibody was labeled with biotin. The presence of targeting antigen HBeAg in blood sample would act as a bridge with biotinylated captured antibody and GNP-conjugated detection antibody to form the dendritic nanoparticle complex. The dendritic complexes in the sample solution were migrated and immobilized on the testing line of strip coated with antibiotin antibodies. Signal intensity was massively amplified by the SAS, which was positively correlated with the concentration of targeting antigen in the blood sample and was assessed by eyes or strip scanner. The SAS worked only when targeting antigens were present in the sample. By using this SAS-LFIA, we were able to detect a very low concentration of HBeAg (9 ng/mL), which was 27-fold sensitive than that by conventional LFIA (cLFIA). A number of 420 blood samples were detested by this novel SAS-LFIA, the results were in accordance with those of enzyme-linked immunosorbent assay (ELISA) completely, while the cLFIA missed an HBeAg-positive sample. In conclusion, the novel SAS has high specificity and sensitivity, which can be used to replace the conventional rapid test and ELISA in clinical diagnosis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Inmunoensayo / Hepatitis E / Oro / Antígenos e de la Hepatitis B Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: J Med Virol Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Inmunoensayo / Hepatitis E / Oro / Antígenos e de la Hepatitis B Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: J Med Virol Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos