Topical administration of hydroethanolic extract of Lawsonia inermis (henna) accelerates excisional wound healing process by reducing tissue inflammation and amplifying glucose uptake.

Daemi, Amin; Farahpour, Mohammad R; Oryan, Ahmad; Karimzadeh, Sirvan; Tajer, Ehsan

Daemi, Amin; Farahpour, Mohammad R; Oryan, Ahmad; Karimzadeh, Sirvan; Tajer, Ehsan.

Afiliación

Daemi A; Department of Biology, Miandoab Branch, Islamic Azad University, Miandoab, Iran.
Farahpour MR; Young Researchers and Elite Club, Urmia Branch, Islamic Azad University, Urmia, Iran.
Oryan A; Department of Pathology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Karimzadeh S; Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran.
Tajer E; Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran.

Kaohsiung J Med Sci ; 35(1): 24-32, 2019 Jan.

Article en En | MEDLINE | ID: mdl-30844141

RESUMEN

Several studies have reported the beneficial effects of Lawsonia inermis on wound healing, but the mechanism of action is still unknown. This study aimed to investigate the effectiveness of a new ointment formulation of hydroethanolic extract leaves of L. inermis on wound healing by gene expression of glucose transporter-1 (Glut-1) and insulin-like growth factor I (Igf-1) in Wistar rats. The animals were topically treated with different doses of L. inermis. An experimentally induced circular excisional wound model of 314 mm2 surface area was surgically created. The percentage of wound contraction and histopathological changes was assessed at different time points following wound induction. The expression of Glut-1 and Igf-1 was evaluated by reverse-transcription PCR. Topical administration of L. inermis, dose dependently, shortened inflammatory phase, accelerated cellular proliferation, and enhanced wound contraction ratio. It also improved revascularization, collagen deposition, and re-epithelialization rate and promoted intracytoplasmic carbohydrate storage (P < 0.05). Moreover, the mRNA levels of Igf-1 and Glut-1 were significantly higher in the L. inermis-treated groups than the control group (P < 0.05). Topical administration of L. inermis promoted the healing process by reducing tissue inflammation and increasing glucose uptake, which was mediated by up-regulating the expression of Igf-1 and Glut-1.

Asunto(s)

Etanol/química; Glucosa/metabolismo; Inflamación/patología; Lawsonia (Planta)/química; Extractos Vegetales/administración & dosificación; Extractos Vegetales/farmacología; Agua/química; Cicatrización de Heridas/efectos de los fármacos; Administración Tópica; Fosfatasa Alcalina/metabolismo; Antioxidantes/metabolismo; Carbohidratos/análisis; Epidermis/efectos de los fármacos; Epidermis/metabolismo; Fibroblastos/efectos de los fármacos; Fibroblastos/patología; Flavonoides/análisis; Transportador de Glucosa de Tipo 1/genética; Transportador de Glucosa de Tipo 1/metabolismo; Factor I del Crecimiento Similar a la Insulina/genética; Factor I del Crecimiento Similar a la Insulina/metabolismo; Queratinocitos/efectos de los fármacos; Queratinocitos/metabolismo; Fenoles/análisis; ARN Mensajero/genética; ARN Mensajero/metabolismo; Repitelización/efectos de los fármacos

Palabras clave

Lawsonia inermis (henna); collagen deposition; excisional wound healing; glucose uptake; tissue inflammation

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cicatrización de Heridas / Agua / Extractos Vegetales / Lawsonia (Planta) / Etanol / Glucosa / Inflamación Tipo de estudio: Prognostic_studies Idioma: En Revista: Kaohsiung J Med Sci Asunto de la revista: MEDICINA Año: 2019 Tipo del documento: Article País de afiliación: Irán Pais de publicación: China

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