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Angiogenic Gene Profiles in Laser-Microdissected Microvessels and Neurons from Ischemic Penumbra of Rat Brain.
Tian, Yingfang; Di, Yuanyuan; Zhang, Jianshui; Chen, Xinlin; Feng, Ting; Adu-Nti, Frank; Shi, Meimei; Fan, Juan; Zhang, Junfeng; Zhang, Pengbo; Liu, Yong.
Afiliación
  • Tian Y; Key Laboratory of Modern Teaching Technology, Ministry of Education, Xi'an, 710062, Shaanxi, China. yingfang_tian@snnu.edu.cn.
  • Di Y; College of Life Sciences, Shaanxi Normal University, Xi'an, 710119, Shaanxi, China. yingfang_tian@snnu.edu.cn.
  • Zhang J; College of Life Sciences, Shaanxi Normal University, Xi'an, 710119, Shaanxi, China.
  • Chen X; Institute of Neurobiology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, 710061, Shaanxi, China.
  • Feng T; Institute of Neurobiology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, 710061, Shaanxi, China.
  • Adu-Nti F; College of Life Sciences, Shaanxi Normal University, Xi'an, 710119, Shaanxi, China.
  • Shi M; College of Life Sciences, Shaanxi Normal University, Xi'an, 710119, Shaanxi, China.
  • Fan J; Key Laboratory of Modern Teaching Technology, Ministry of Education, Xi'an, 710062, Shaanxi, China.
  • Zhang J; College of Life Sciences, Shaanxi Normal University, Xi'an, 710119, Shaanxi, China.
  • Zhang P; Department of Anatomy, Xi'an Medical University, Xi'an, 710021, Shaanxi, China.
  • Liu Y; Department of Anesthesia of the Second Affiliated Hospital, Xi'an Jiaotong University Health Science Center, Xi'an, 710004, Shaanxi, China.
J Mol Neurosci ; 67(4): 643-653, 2019 Apr.
Article en En | MEDLINE | ID: mdl-30840225
Angiogenesis is induced immediately after cerebral ischemia and plays a pivotal role in the strategy against ischemic injury. We hypothesized that the coordinated interaction between microvessels and neurons was altered immediately after stroke, and microvessels and neurons would show the temporal specificity of angiogenic gene profiles after cerebral ischemia. Microvessels and neurons were harvested in the ischemic penumbra of rat brain using the PixCell II laser capture microdissection (LCM) instrument. After RNA isolation, T7 and gene-specific primer RNA linear amplification were performed, and angiogenic functional grouping cDNA profiling was analyzed in LCM samples. cDNA microarray results showed there were 35 (36.46%) and 27 (28.13%) genes expression changes in the microvessels, while 25 (26.04%) and 31 (32.29%) genes were changed in the neurons at 2 h and 24 h after cerebral ischemia. Members of growth factors and receptors, cytokines and chemokines, adhesion molecules, matrix proteins, proteases, and inhibitors showed temporal and spatial differentiation in the microvessels and neurons after cerebral ischemia. This finding will help to understand the coordination and interaction between microvessels and neurons, and to elucidate the molecular mechanisms of angiogenesis after brain ischemic injury.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neovascularización Fisiológica / Infarto de la Arteria Cerebral Media / Microvasos / Transcriptoma / Neuronas Límite: Animals Idioma: En Revista: J Mol Neurosci Asunto de la revista: BIOLOGIA MOLECULAR / NEUROLOGIA Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neovascularización Fisiológica / Infarto de la Arteria Cerebral Media / Microvasos / Transcriptoma / Neuronas Límite: Animals Idioma: En Revista: J Mol Neurosci Asunto de la revista: BIOLOGIA MOLECULAR / NEUROLOGIA Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos