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A urinary microRNA panel that is an early predictive biomarker of delayed graft function following kidney transplantation.
Khalid, Usman; Newbury, Lucy J; Simpson, Kate; Jenkins, Robert H; Bowen, Timothy; Bates, Lucy; Sheerin, Neil S; Chavez, Rafael; Fraser, Donald J.
Afiliación
  • Khalid U; Wales Kidney Research Unit, School of Medicine, College of Biomedical & Life Sciences, Cardiff University, Heath Park Campus, Cardiff, CF14 4XN, UK.
  • Newbury LJ; Cardiff Transplant Unit, University Hospital of Wales, Cardiff, CF14 4XW, UK.
  • Simpson K; Wales Kidney Research Unit, School of Medicine, College of Biomedical & Life Sciences, Cardiff University, Heath Park Campus, Cardiff, CF14 4XN, UK.
  • Jenkins RH; Wales Kidney Research Unit, School of Medicine, College of Biomedical & Life Sciences, Cardiff University, Heath Park Campus, Cardiff, CF14 4XN, UK.
  • Bowen T; Wales Kidney Research Unit, School of Medicine, College of Biomedical & Life Sciences, Cardiff University, Heath Park Campus, Cardiff, CF14 4XN, UK.
  • Bates L; Wales Kidney Research Unit, School of Medicine, College of Biomedical & Life Sciences, Cardiff University, Heath Park Campus, Cardiff, CF14 4XN, UK.
  • Sheerin NS; Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.
  • Chavez R; Newcastle upon Tyne Hospitals NHS Foundation Trust, Newcastle upon Tyne, UK.
  • Fraser DJ; Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.
Sci Rep ; 9(1): 3584, 2019 03 05.
Article en En | MEDLINE | ID: mdl-30837502
Predicting immediate and subsequent graft function is important in clinical decision-making around kidney transplantation, but is difficult using available approaches. Here we have evaluated urinary microRNAs as biomarkers in this context. Profiling of 377 microRNAs in the first urine passed post-transplantation identified 6 microRNAs, confirmed to be upregulated by RT-qPCR in an expanded cohort (miR-9, -10a, -21, -29a, -221, and -429, n = 33, P < 0.05 for each). Receiver operating characteristic analysis showed Area Under the Curve 0.94 for this panel. To establish whether this early signal was sustained, miR-21 was measured daily for 5 days post-transplant, and was consistently elevated in those developing Delayed Graft Function (n = 165 samples from 33 patients, p < 0.05). The biomarker panel was then evaluated in an independent cohort, sampled at varying times in the first week post-transplantation in a separate transplant center. When considered individually, all miRs in the panel showed a trend to increase or a significant increase in those developing delayed Graft Function (miR-9: P = 0.068, mIR-10a: P = 0.397, miR-21: P = 0.003, miR-29a: P = 0.019, miR-221: P = 0.1, and miR-429: P = 0.013, n = 47) with Area Under the Curve 0.75 for the panel. In conclusion, combined measurement of six microRNAs had predictive value for delayed graft function following kidney transplantation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trasplante de Riñón / MicroARNs / Funcionamiento Retardado del Injerto Tipo de estudio: Etiology_studies / Incidence_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Límite: Adolescent / Adult / Aged / Child / Female / Humans / Male / Middle aged Idioma: En Revista: Sci Rep Año: 2019 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trasplante de Riñón / MicroARNs / Funcionamiento Retardado del Injerto Tipo de estudio: Etiology_studies / Incidence_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Límite: Adolescent / Adult / Aged / Child / Female / Humans / Male / Middle aged Idioma: En Revista: Sci Rep Año: 2019 Tipo del documento: Article Pais de publicación: Reino Unido