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Alternative splicing regulation of doublesex gene by RNA-binding proteins in the silkworm Bombyx mori.
Zheng, Zeng-Zhang; Sun, Xia; Zhang, Bei; Pu, Jia; Jiang, Ze-Yu; Li, Muwang; Fan, Yu-Jie; Xu, Yong-Zhen.
Afiliación
  • Zheng ZZ; a Key Laboratory of Insect Developmental and Evolutionary Biology, CAS Center for Excellence in Molecular Plant Science , Institute of Plant Physiology and Ecology, Chinese Academy of Sciences , Shanghai , China.
  • Sun X; b College of Life Technology , Jiangsu University of Science and Technology , Zhenjiang , China.
  • Zhang B; a Key Laboratory of Insect Developmental and Evolutionary Biology, CAS Center for Excellence in Molecular Plant Science , Institute of Plant Physiology and Ecology, Chinese Academy of Sciences , Shanghai , China.
  • Pu J; a Key Laboratory of Insect Developmental and Evolutionary Biology, CAS Center for Excellence in Molecular Plant Science , Institute of Plant Physiology and Ecology, Chinese Academy of Sciences , Shanghai , China.
  • Jiang ZY; a Key Laboratory of Insect Developmental and Evolutionary Biology, CAS Center for Excellence in Molecular Plant Science , Institute of Plant Physiology and Ecology, Chinese Academy of Sciences , Shanghai , China.
  • Li M; b College of Life Technology , Jiangsu University of Science and Technology , Zhenjiang , China.
  • Fan YJ; c College of Life Science , Wuhan University , Wuhan , China.
  • Xu YZ; c College of Life Science , Wuhan University , Wuhan , China.
RNA Biol ; 16(6): 809-820, 2019 06.
Article en En | MEDLINE | ID: mdl-30836863
Doublesex is highly conserved and sex-specifically spliced in insect sex-determination pathways, and its alternative splicing (AS) is regulated by Transformer, an exonic splicing activator, in the model system of Drosophila melanogaster. However, due to the lack of a transformer gene, AS regulation of doublesex remains unclear in Lepidoptera, which contain the economically important silkworm Bombyx mori and thousands of agricultural pests. Here, we use yeast three-hybrid system to screen for RNA-binding proteins that recognize sex-specific exons 3 and 4 of silkworm doublesex (Bm-dsx); this approach identified BxRBP1/Lark binding to the exon 3, and BxRBP2/TBPH and BxRBP3/Aret binding to the exon 4. Investigation of tissues shows that BxRBP1 and BxRBP2 have no sex specificity, but BxRBP3 has - three of its four isoforms are expressed with a sex-bias. Using novel sex-specific silkworm cell lines, we find that BxRBP1 and BxRBP3 directly interact with each other, and cooperatively function as splicing repressors. Over-expression of BxRBP1 and BxRBP3 isoforms efficiently inhibits splicing of the exons 3 and 4 in the female-specific cells and generates the male-specific isoform of Bm-dsx. We also demonstrate that the sex-determination upstream gene Masc regulates alternatively transcribed BxRBP3 isoforms. Thus, we identify a new regulatory mechanism of doublesex AS in the silkworm, revealing an evolutionary divergence in insect sex-determination.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bombyx / Proteínas de Unión al ARN / Empalme Alternativo / Proteínas de Insectos / Proteínas de Unión al ADN Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: RNA Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bombyx / Proteínas de Unión al ARN / Empalme Alternativo / Proteínas de Insectos / Proteínas de Unión al ADN Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: RNA Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos