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Diversity of Cryptosporidium spp. in Apodemus spp. in Europe.
Condlová, Sárka; Horcicková, Michaela; Havrdová, Nikola; Sak, Bohumil; Hlásková, Lenka; Perec-Matysiak, Agnieszka; Kicia, Marta; McEvoy, John; Kvác, Martin.
Afiliación
  • Condlová S; Faculty of Agriculture, University of South Bohemia in Ceské Budejovice, Czech Republic; Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Ceské Budejovice, Czech Republic.
  • Horcicková M; Faculty of Agriculture, University of South Bohemia in Ceské Budejovice, Czech Republic; Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Ceské Budejovice, Czech Republic.
  • Havrdová N; Faculty of Agriculture, University of South Bohemia in Ceské Budejovice, Czech Republic; Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Ceské Budejovice, Czech Republic.
  • Sak B; Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Ceské Budejovice, Czech Republic.
  • Hlásková L; Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Ceské Budejovice, Czech Republic.
  • Perec-Matysiak A; Department of Parasitology, Institute of Genetics and Microbiology, University of Wroclaw, Poland.
  • Kicia M; Department of Biology and Medical Parasitology, Wroclaw Medical University, Poland.
  • McEvoy J; Microbiological Sciences Department, North Dakota State University, Fargo, USA.
  • Kvác M; Faculty of Agriculture, University of South Bohemia in Ceské Budejovice, Czech Republic; Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Ceské Budejovice, Czech Republic. Electronic address: kvac@paru.cas.cz.
Eur J Protistol ; 69: 1-13, 2019 Jun.
Article en En | MEDLINE | ID: mdl-30826667
The genetic diversity of Cryptosporidium spp. in Apodemus spp. (striped field mouse, yellow-necked mouse and wood mouse) from 16 European countries was examined by PCR/sequencing of isolates from 437 animals. Overall, 13.7% (60/437) of animals were positive for Cryptosporidium by PCR. Phylogenetic analysis of small-subunit rRNA, Cryptosporidium oocyst wall protein and actin gene sequences showed the presence of Cryptosporidium ditrichi (22/60), Cryptosporidium apodemi (13/60), Cryptosporidium apodemus genotype I (8/60), Cryptosporidium apodemus genotype II (9/60), Cryptosporidium parvum (2/60), Cryptosporidium microti (2/60), Cryptosporidium muris (2/60) and Cryptosporidium tyzzeri (2/60). At the gp60 locus, novel gp60 families XVIIa and XVIIIa were identified in Cryptosporidium apodemus genotype I and II, respectively, subtype IIaA16G1R1b was identified in C. parvum, and subtypes IXaA8 and IXcA6 in C. tyzzeri. Only animals infected with C. ditrichi, C. apodemi, and Cryptosporidium apodemus genotypes shed oocysts that were detectable by microscopy, with the infection intensity ranging from 2000 to 52,000 oocysts per gram of faeces. None of the faecal samples was diarrheic in the time of the sampling.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Variación Genética / Criptosporidiosis / Cryptosporidium / Murinae Límite: Animals País/Región como asunto: Europa Idioma: En Revista: Eur J Protistol Asunto de la revista: BIOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: República Checa Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Variación Genética / Criptosporidiosis / Cryptosporidium / Murinae Límite: Animals País/Región como asunto: Europa Idioma: En Revista: Eur J Protistol Asunto de la revista: BIOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: República Checa Pais de publicación: Alemania