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Platelet-Rich Fibrin Extract: A Promising Fetal Bovine Serum Alternative in Explant Cultures of Human Periosteal Sheets for Regenerative Therapy.
Kawase, Tomoyuki; Nagata, Masaki; Okuda, Kazuhiro; Ushiki, Takashi; Fujimoto, Yoko; Watanabe, Mari; Ito, Akira; Nakata, Koh.
Afiliación
  • Kawase T; Division of Oral Bioengineering, Institute of Medicine and Dentistry, Niigata University, Niigata 951-8514, Japan. kawase@dent.niigata-u.ac.jp.
  • Nagata M; Division of Oral Surgery, Institute of Medicine and Dentistry, Niigata University, Niigata 951-8514, Japan. nagatam@dent.niigata-u.ac.jp.
  • Okuda K; Division of Periodontology, Institute of Medicine and Dentistry, Niigata University, Niigata 951-8514, Japan. okuda@dent.niigata-u.ac.jp.
  • Ushiki T; Bioscience Medical Research Center, Niigata University Medical and Dental Hospital, Niigata 951-8520, Japan. tushiki@med.niigata-u.ac.jp.
  • Fujimoto Y; Bioscience Medical Research Center, Niigata University Medical and Dental Hospital, Niigata 951-8520, Japan. yfujimoto@med.niigata-u.ac.jp.
  • Watanabe M; Bioscience Medical Research Center, Niigata University Medical and Dental Hospital, Niigata 951-8520, Japan. mwatanabe@med.niigata-u.ac.jp.
  • Ito A; Kohjin Bio Co., Ltd., Sakado 350-0214, Japan. a.ito@kohjin-bio.co.jp.
  • Nakata K; Bioscience Medical Research Center, Niigata University Medical and Dental Hospital, Niigata 951-8520, Japan. radical@med.niigata-u.ac.jp.
Int J Mol Sci ; 20(5)2019 Feb 28.
Article en En | MEDLINE | ID: mdl-30823423
In 2004, we developed autologous periosteal sheets for the treatment of periodontal bone defects. This regenerative therapy has successfully regenerated periodontal bone and augmented alveolar ridge for implant placement. However, the necessity for 6-week culture is a limitation. Here, we examined the applicability of a human platelet-rich fibrin extract (PRFext) as an alternative to fetal bovine serum (FBS) for the explant culture of periosteal sheets in a novel culture medium (MSC-PCM) originally developed for maintaining mesenchymal stem cells. Small periosteum tissue segments were expanded in MSC-PCM + 2% PRFext for 4 weeks, and the resulting periosteal sheets were compared with those prepared by the conventional method using Medium199 + 10% FBS for their growth rate, cell multilayer formation, alkaline phosphatase (ALP) activity, and surface antigen expression (CD73, CD90, and CD105). Periosteal sheets grew faster in the novel culture medium than in the conventional medium. However, assessment of cell shape and ALP activity revealed that the periosteal cells growing in the novel medium were relatively immature. These findings suggest that the novel culture medium featuring PRFext offers advantages by shortening the culture period and excluding possible risks associated with xeno-factors without negatively altering the activity of periosteal sheets.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Periostio / Medios de Cultivo / Medicina Regenerativa / Técnicas de Cultivo de Tejidos / Fibrina Rica en Plaquetas Límite: Adult / Female / Humans / Male Idioma: En Revista: Int J Mol Sci Año: 2019 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Periostio / Medios de Cultivo / Medicina Regenerativa / Técnicas de Cultivo de Tejidos / Fibrina Rica en Plaquetas Límite: Adult / Female / Humans / Male Idioma: En Revista: Int J Mol Sci Año: 2019 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Suiza