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RECQL5: Another DNA helicase potentially involved in hereditary breast cancer susceptibility.
Tavera-Tapia, Alejandra; de la Hoya, Miguel; Calvete, Oriol; Martin-Gimeno, Paloma; Fernández, Victoria; Macías, José Antonio; Alonso, Beatriz; Pombo, Luz; de Diego, Carles; Alonso, Rosario; Pita, Guillermo; Barroso, Alicia; Urioste, Miguel; Caldés, Trinidad; Newman, Joseph A; Benítez, Javier; Osorio, Ana.
Afiliación
  • Tavera-Tapia A; Human Genetics Group, Human Cancer Genetics Programme, Spanish National Cancer Research Centre, Madrid, Spain.
  • de la Hoya M; Molecular Oncology Laboratory (CIBERONC), Hospital Clínico San Carlos, IdISSC, Madrid, Spain.
  • Calvete O; Human Genetics Group, Human Cancer Genetics Programme, Spanish National Cancer Research Centre, Madrid, Spain.
  • Martin-Gimeno P; Human Genetics Group, Human Cancer Genetics Programme, Spanish National Cancer Research Centre, Madrid, Spain.
  • Fernández V; Spanish Network on Rare Diseases (CIBERER), Madrid, Spain.
  • Macías JA; Human Genetics Group, Human Cancer Genetics Programme, Spanish National Cancer Research Centre, Madrid, Spain.
  • Alonso B; Hereditary Cancer Unit, Medical Oncology Service, Hospital Morales Messeguer, Murcia, Spain.
  • Pombo L; Medical Oncology Service, University Hospital of Canarias, La Laguna, Santa Cruz, Spain.
  • de Diego C; Medical Oncology Section, University Hospital Complex of Albacete, Spain.
  • Alonso R; Genetics Service, Virgen de la Salud Hospital, Toledo, Spain.
  • Pita G; Genotyping Unit, CEGEN, Human Cancer Genetics Programme, Spanish National Cancer Research Centre, Madrid, Spain.
  • Barroso A; Genotyping Unit, CEGEN, Human Cancer Genetics Programme, Spanish National Cancer Research Centre, Madrid, Spain.
  • Urioste M; Human Genetics Group, Human Cancer Genetics Programme, Spanish National Cancer Research Centre, Madrid, Spain.
  • Caldés T; Spanish Network on Rare Diseases (CIBERER), Madrid, Spain.
  • Newman JA; Familial Cancer Clinical Unit, Human Cancer Genetics Programme, Spanish National Cancer Research Centre, Madrid, Spain.
  • Benítez J; Molecular Oncology Laboratory (CIBERONC), Hospital Clínico San Carlos, IdISSC, Madrid, Spain.
  • Osorio A; Structural Genomics Consortium, University of Oxford, ORCRB, Oxford, UK.
Hum Mutat ; 40(5): 566-577, 2019 05.
Article en En | MEDLINE | ID: mdl-30817846
There is still around 50% of the familial breast cancer (BC) cases with an undefined genetic cause, here we have used next-generation sequencing (NGS) technology to identify new BC susceptibility genes. This approach has led to the identification of RECQL5, a member of RECQL-helicases family, as a new BC susceptibility candidate, which deserves further study. We have used a combination of whole exome sequencing in a family negative for mutations in BRCA1/2 throughout (BRCAX), in which we found a probably deleterious variant in RECQL5, and targeted NGS of the complete coding regions and exon-intron boundaries of the candidate gene in 699 BC Spanish BRCAX families and 665 controls. Functional characterization and in silico inference of pathogenicity were performed to evaluate the deleterious effect of detected variants. We found at least seven deleterious or likely deleterious variants among the cases and only one in controls. These results prompt us to propose RECQL5 as a gene that would be worth to analyze in larger studies to explore its possible implication in BC susceptibility.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Mama / Predisposición Genética a la Enfermedad / RecQ Helicasas Límite: Female / Humans Idioma: En Revista: Hum Mutat Asunto de la revista: GENETICA MEDICA Año: 2019 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Mama / Predisposición Genética a la Enfermedad / RecQ Helicasas Límite: Female / Humans Idioma: En Revista: Hum Mutat Asunto de la revista: GENETICA MEDICA Año: 2019 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos