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Novel type of hollow hydrogel microspheres with magnetite and silver nanoparticles.
Lengert, Ekaterina; Kozlova, Anastasiia; Pavlov, Anton M; Atkin, Vsevolod; Verkhovskii, Roman; Kamyshinsky, Roman; Demina, Polina; Vasiliev, Alexander L; Venig, Sergey B; Bukreeva, Tatyana V.
Afiliación
  • Lengert E; Shubnikov Institute of Crystallography, Russian Academy of Sciences, 59 Leninskii pr., Moscow, 119333, Russia; Department of Nano- and Biomedical Technologies, Saratov State University, 83 Astrakhanskaya st., 410012 Saratov, Russia; Department of Molecular Biotechnology, Ghent University, Coupure Li
  • Kozlova A; Department of Nano- and Biomedical Technologies, Saratov State University, 83 Astrakhanskaya st., 410012 Saratov, Russia.
  • Pavlov AM; Department of Nano- and Biomedical Technologies, Saratov State University, 83 Astrakhanskaya st., 410012 Saratov, Russia. Electronic address: pavlovam@sgu.ru.
  • Atkin V; Department of Nano- and Biomedical Technologies, Saratov State University, 83 Astrakhanskaya st., 410012 Saratov, Russia.
  • Verkhovskii R; Department of Nano- and Biomedical Technologies, Saratov State University, 83 Astrakhanskaya st., 410012 Saratov, Russia; School of Urbanistics, Civil Engineering and Architecture Chair of Ecology, Yuri Gagarin State Technical University of Saratov.
  • Kamyshinsky R; National Research Center "Kurchatov Institute", Akademika Kurchatova pl., 1, 123182 Moscow, Russia; Moscow Institute of Physics and Technology, 9 Institutsky lane, Dolgoprudny, 141700, Moscow Region, Russia.
  • Demina P; Shubnikov Institute of Crystallography, Russian Academy of Sciences, 59 Leninskii pr., Moscow, 119333, Russia; Department of Nano- and Biomedical Technologies, Saratov State University, 83 Astrakhanskaya st., 410012 Saratov, Russia.
  • Vasiliev AL; National Research Center "Kurchatov Institute", Akademika Kurchatova pl., 1, 123182 Moscow, Russia; Moscow Institute of Physics and Technology (State University), MIPT, 9 Institutskiy per., Dolgoprudny, 141701 Moscow Region, Russia.
  • Venig SB; Department of Nano- and Biomedical Technologies, Saratov State University, 83 Astrakhanskaya st., 410012 Saratov, Russia.
  • Bukreeva TV; Shubnikov Institute of Crystallography, Russian Academy of Sciences, 59 Leninskii pr., Moscow, 119333, Russia; National Research Center "Kurchatov Institute", Akademika Kurchatova pl., 1, 123182 Moscow, Russia.
Mater Sci Eng C Mater Biol Appl ; 98: 1114-1121, 2019 May.
Article en En | MEDLINE | ID: mdl-30812995
A novel type of microcontainers based on hollow silver alginate microspheres and magnetite nanoparticles is reported as development of recently published technology. Magnetite nanoparticles were incorporated by two methods - co-precipitation with porous calcium carbonate during template formation and adsorption onto CaCO3 particles or microcontainers' shell. Amount of magnetite loaded and microshells size (4.6 to 6.9 µm) were found to depend on the chosen method for magnetite nanoparticles incorporation. Stability of hollow microshells in saline, phosphate buffer and culturing media was studied. Microcontainers' susceptibility to magnetic field was investigated in solutions of varied viscosity, and their group movement velocity under constant magnetic field was evaluated by sequential optical microscopy imaging. Cell viability tests with prepared microshells were performed that demonstrated negligible cytotoxicity effect on human dermal fibroblasts cells. With HeLa cells moderate viability inhibition was found at high carriers:cells ratio at early time points which is attributed to more active and receptor-mediated endocytosis of carriers as well as known cytotoxicity of magnetite in some cancer cells. At 24 and 48 h time points HeLa cells proliferation fully recovers. Reported data opens perspectives for further biomedical-oriented studies and application of this novel kind of microcontainers with a number of techniques applicable for imaging, control and triggered cargo release provided by presence of silver and magnetite nanoparticles in the carriers and their suitability for further versatile functionalization by traditional LbL approach if needed.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plata / Hidrogeles / Óxido Ferrosoférrico / Nanopartículas de Magnetita Límite: Humans Idioma: En Revista: Mater Sci Eng C Mater Biol Appl Año: 2019 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plata / Hidrogeles / Óxido Ferrosoférrico / Nanopartículas de Magnetita Límite: Humans Idioma: En Revista: Mater Sci Eng C Mater Biol Appl Año: 2019 Tipo del documento: Article Pais de publicación: Países Bajos