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Determination of the Critical Concentration of Rifabutin for Susceptibility Testing Using the Proportion Method with Löwenstein-Jensen Medium against Mycobacterium Tuberculosis Isolates.
Dai, Guangming; Chen, Suting; Dong, Lingling; Li, Yun Xu; Huo, Fengmin; Shang, Yuanyuan; Huang, Hairong.
Afiliación
  • Dai G; National Clinical Laboratory on Tuberculosis, Beijing Key Laboratory for Drug Resistance Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing, China.
  • Chen S; National Clinical Laboratory on Tuberculosis, Beijing Key Laboratory for Drug Resistance Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing, China.
  • Dong L; National Clinical Laboratory on Tuberculosis, Beijing Key Laboratory for Drug Resistance Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing, China.
  • Li YX; National Clinical Laboratory on Tuberculosis, Beijing Key Laboratory for Drug Resistance Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing, China.
  • Huo F; National Clinical Laboratory on Tuberculosis, Beijing Key Laboratory for Drug Resistance Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing, China.
  • Shang Y; National Clinical Laboratory on Tuberculosis, Beijing Key Laboratory for Drug Resistance Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing, China.
  • Huang H; National Clinical Laboratory on Tuberculosis, Beijing Key Laboratory for Drug Resistance Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing, China.
Lab Med ; 50(3): 292-297, 2019 Jul 16.
Article en En | MEDLINE | ID: mdl-30806669
OBJECTIVE: To determine the critical concentration of rifabutin (RFB) for susceptibility testing against Mycobacterium tuberculosis (Mtb) on Löwenstein-Jensen (L-J) medium using the proportion method. METHODS: We used 47 strains were used to determine the critical concentration of RFB. The microplate antimicrobial assay (MABA) was used as a reference method. We used 160 strains to evaluate its correlation with the classification results derived from the MABA method. We performed antimicrobial susceptibility testing (AST) against RFB and rifampin (RIF) for 2748 other strains using the proportion method on L-J medium. RESULTS: The determined critical concentration for RFB was 20 µg per mL. Identical classification as susceptible or resistant was observed in 93.8% and 92.5% strains for RFB and RIF, respectively, using the 2 different methods. The cross-resistance ratio between RFB and RIF was 72.7% in the 2748 Mtb strains. CONCLUSIONS: We determined that a critical concentration of 20 µg per mL RFB was reliable for AST of Mtb.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pruebas de Sensibilidad Microbiana / Rifabutina / Medios de Cultivo / Antibióticos Antituberculosos / Mycobacterium tuberculosis Idioma: En Revista: Lab Med Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pruebas de Sensibilidad Microbiana / Rifabutina / Medios de Cultivo / Antibióticos Antituberculosos / Mycobacterium tuberculosis Idioma: En Revista: Lab Med Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido