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Spermatological Morphology of Triatoma Species (Hemiptera: Reduviidae: Triatominae).
Oliveira, Maria Luiza Ribeiro de; Camara, Daniel Cardoso Portela; Freitas, Simone Patrícia Carneiro; Santos-Mallet, Jacenir Reis.
Afiliación
  • Oliveira MLR; Laboratório Interdisciplinar de Vigilância Entomológica em Diptera e Hemiptera, Instituto Oswaldo Cruz, FIOCRUZ. Av. Brasil 4365. Pavilhão Carlos Chagas, 5º andar. Rio de Janeiro, RJ, Brasil.
  • Camara DCP; Programa de Pós-graduação em Biologia Animal, Universidade Federal Rural do Estado do Rio de Janeiro. Rodovia BR 465, Km 07, s/n. Zona Rural. Seropédica, RJ, Brasil.
  • Freitas SPC; Laboratório de Mosquitos Transmissores de Hematozoários, Instituto Oswaldo Cruz, FIOCRUZ, Av. Brasil, 4365, Pavilhão Carlos Chagas, 4º andar, Rio de Janeiro, RJ, Brasil.
  • Santos-Mallet JR; Laboratório Interdisciplinar de Vigilância Entomológica em Diptera e Hemiptera, Instituto Oswaldo Cruz, FIOCRUZ. Av. Brasil 4365. Pavilhão Carlos Chagas, 5º andar. Rio de Janeiro, RJ, Brasil.
J Med Entomol ; 56(4): 959-966, 2019 06 27.
Article en En | MEDLINE | ID: mdl-30801646
Spermatozoa are among the most diverse cell types, and their morphologies often provide data that can be used to reliably evaluate phylogenetic relationships. They can also help to clarify the nature of 'specific complexes', which are common among triatomines. In the present study, we evaluated the copulation behavior of Triatoma rubrovaria Blanchard 1843 (Hemiptera: Reduviidae: Triatominae) and the structural morphology of sperm from T. carcavalloi Jurberg Rocha & Lent, 1998, T. infestans Klug, 1834, T. pintodiasi Jurberg Cunha & Rocha, 2013, and T. rubrovaria. Copulatory behavior was described from the moment males and females genitalia joined until they separated. Insemination was confirmed by the presence of a spermatophore in the female's bursa copulatrix. To measure their sperm, males were dissected and their seminal vesicles were removed, squashed on glass slides, and then spread, fixed, and observed under a photomicroscope. The images obtained were analyzed to measure the sperm. Seminal vesicles were also prepared for transmission electron microscopy. We performed K-means clustering separately for each species to group their sperm based on morphology. The differences in spermatozoa length among species of Triatominae, sperm types, and the interaction between species and sperm type were assessed with a two-way analysis of variance (ANOVA). The copulation time in T. rubrovaria was 3 to 5 min, which was sufficiently long for spermatophore transfer. All taxa showed polymorphic (short and long) sperm, with significant differences in the lengths of sperm among taxa. Using electron microscopy, the sperm cells of the four taxa examined were found to have similar ultrastructural morphology, confirming the hypothesized synapomorphies of sperm within the suborder Heteroptera (Hemiptera).
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espermatozoides / Triatoma Límite: Animals Idioma: En Revista: J Med Entomol Año: 2019 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espermatozoides / Triatoma Límite: Animals Idioma: En Revista: J Med Entomol Año: 2019 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido