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Aurora A depletion reveals centrosome-independent polarization mechanism in Caenorhabditis elegans.
Klinkert, Kerstin; Levernier, Nicolas; Gross, Peter; Gentili, Christian; von Tobel, Lukas; Pierron, Marie; Busso, Coralie; Herrman, Sarah; Grill, Stephan W; Kruse, Karsten; Gönczy, Pierre.
Afiliación
  • Klinkert K; Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology Lausanne (EPFL), Lausanne, Switzerland.
  • Levernier N; Department of Biochemistry, University of Geneva, Geneva, Switzerland.
  • Gross P; Department of Theoretical Physics, University of Geneva, Geneva, Switzerland.
  • Gentili C; BIOTEC, TU Dresden, Dresden, Germany.
  • von Tobel L; Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology Lausanne (EPFL), Lausanne, Switzerland.
  • Pierron M; Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology Lausanne (EPFL), Lausanne, Switzerland.
  • Busso C; Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology Lausanne (EPFL), Lausanne, Switzerland.
  • Herrman S; Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology Lausanne (EPFL), Lausanne, Switzerland.
  • Grill SW; Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology Lausanne (EPFL), Lausanne, Switzerland.
  • Kruse K; BIOTEC, TU Dresden, Dresden, Germany.
  • Gönczy P; Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
Elife ; 82019 02 26.
Article en En | MEDLINE | ID: mdl-30801250
How living systems break symmetry in an organized manner is a fundamental question in biology. In wild-type Caenorhabditis elegans zygotes, symmetry breaking during anterior-posterior axis specification is guided by centrosomes, resulting in anterior-directed cortical flows and a single posterior PAR-2 domain. We uncover that C. elegans zygotes depleted of the Aurora A kinase AIR-1 or lacking centrosomes entirely usually establish two posterior PAR-2 domains, one at each pole. We demonstrate that AIR-1 prevents symmetry breaking early in the cell cycle, whereas centrosomal AIR-1 instructs polarity initiation thereafter. Using triangular microfabricated chambers, we establish that bipolarity of air-1(RNAi) embryos occurs effectively in a cell-shape and curvature-dependent manner. Furthermore, we develop an integrated physical description of symmetry breaking, wherein local PAR-2-dependent weakening of the actin cortex, together with mutual inhibition of anterior and posterior PAR proteins, provides a mechanism for spontaneous symmetry breaking without centrosomes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Caenorhabditis elegans / Centrosoma / Tipificación del Cuerpo / Proteínas de Caenorhabditis elegans / Aurora Quinasa A Límite: Animals Idioma: En Revista: Elife Año: 2019 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Caenorhabditis elegans / Centrosoma / Tipificación del Cuerpo / Proteínas de Caenorhabditis elegans / Aurora Quinasa A Límite: Animals Idioma: En Revista: Elife Año: 2019 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Reino Unido