Intensive Distribution of G2-Quaduplexes in the Pseudorabies Virus Genome and Their Sensitivity to Cations and G-Quadruplex Ligands.

Deng, Hui; Gong, Bowen; Yang, Zhiquan; Li, Zhen; Zhou, Huan; Zhang, Yashu; Niu, Xiaohui; Liu, Sisi; Wei, Dengguo

Deng, Hui; Gong, Bowen; Yang, Zhiquan; Li, Zhen; Zhou, Huan; Zhang, Yashu; Niu, Xiaohui; Liu, Sisi; Wei, Dengguo.

Afiliación

Deng H; Laboratory of Medicinal Biophysical Chemistry, College of Science, Huazhong Agricultural University, Wuhan 430070, China. denghui0923@163.com.
Gong B; Laboratory of Medicinal Biophysical Chemistry, College of Science, Huazhong Agricultural University, Wuhan 430070, China. night@webmail.hzau.edu.cn.
Yang Z; Hubei Key Laboratory of Agricultural Bioinformatics, College of Informatics, Huazhong Agricultural University, Wuhan 430070, China. yang_zq@foxmail.com.
Li Z; Laboratory of Medicinal Biophysical Chemistry, College of Science, Huazhong Agricultural University, Wuhan 430070, China. lizhen5a@163.com.
Zhou H; Laboratory of Medicinal Biophysical Chemistry, College of Science, Huazhong Agricultural University, Wuhan 430070, China. zhouhuan@webmail.hzau.edu.cn.
Zhang Y; Laboratory of Medicinal Biophysical Chemistry, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. yashu@webmail.hzau.edu.cn.
Niu X; Hubei Key Laboratory of Agricultural Bioinformatics, College of Informatics, Huazhong Agricultural University, Wuhan 430070, China. niuxiaoh@126.com.
Liu S; Laboratory of Medicinal Biophysical Chemistry, College of Science, Huazhong Agricultural University, Wuhan 430070, China. liusisi@mail.hzau.edu.cn.
Wei D; Laboratory of Medicinal Biophysical Chemistry, College of Science, Huazhong Agricultural University, Wuhan 430070, China. dgwei@mail.hzau.edu.cn.

Molecules ; 24(4)2019 Feb 21.

Article en En | MEDLINE | ID: mdl-30795541

RESUMEN

Guanine-rich sequences in the genomes of herpesviruses can fold into G-quadruplexes. Compared with the widely-studied G3-quadruplexes, the dynamic G2-quadruplexes are more sensitive to the cell microenvironment, but they attract less attention. Pseudorabies virus (PRV) is the model species for the study of the latency and reactivation of herpesvirus in the nervous system. A total of 1722 G2-PQSs and 205 G3-PQSs without overlap were identified in the PRV genome. Twelve G2-PQSs from the CDS region exhibited high conservation in the genomes of the Varicellovirus genus. Eleven G2-PQSs were 100% conserved in the repeated region of the annotated PRV genomes. There were 212 non-redundant G2-PQSs in the 3' UTR and 19 non-redundant G2-PQSs in the 5' UTR, which would mediate gene expression in the post-transcription and translation processes. The majority of examined G2-PQSs formed parallel structures and exhibited different sensitivities to cations and small molecules in vitro. Two G2-PQSs, respectively, from 3' UTR of UL5 (encoding helicase motif) and UL9 (encoding sequence-specific ori-binding protein) exhibited diverse regulatory activities with/without specific ligands in vivo. The G-quadruplex ligand, NMM, exhibited a potential for reducing the virulence of the PRV Ea strain. The systematic analysis of the distribution of G2-PQSs in the PRV genomes could guide further studies of the G-quadruplexes' functions in the life cycle of herpesviruses.

Asunto(s)

ADN Viral/química; G-Cuádruplex/efectos de los fármacos; Regulación Viral de la Expresión Génica; Genoma Viral; Herpesvirus Suido 1/genética; Regiones no Traducidas 3'/efectos de los fármacos; Regiones no Traducidas 5'/efectos de los fármacos; Acridinas/química; Acridinas/farmacología; Aminoquinolinas/química; Aminoquinolinas/farmacología; Animales; Bovinos; Línea Celular; Biología Computacional/métodos; ADN Helicasas/genética; ADN Helicasas/metabolismo; ADN Primasa/genética; ADN Primasa/metabolismo; ADN Viral/genética; ADN Viral/metabolismo; Células Epiteliales/efectos de los fármacos; Células Epiteliales/virología; Células HEK293; Herpesvirus Suido 1/efectos de los fármacos; Herpesvirus Suido 1/metabolismo; Humanos; Ligandos; Mesoporfirinas/química; Mesoporfirinas/farmacología; Ácidos Picolínicos/química; Ácidos Picolínicos/farmacología; Regiones Promotoras Genéticas/efectos de los fármacos; Isoformas de Proteínas/genética; Isoformas de Proteínas/metabolismo; Porcinos; Varicellovirus/efectos de los fármacos; Varicellovirus/genética; Varicellovirus/metabolismo; Proteínas Virales/genética; Proteínas Virales/metabolismo; Activación Viral/efectos de los fármacos; Replicación Viral/efectos de los fármacos

Palabras clave

G-quadruplex; G-quadruplex ligand; alphaherpesviruses; genome; nucleic acids conformation; pseudorabies virus; regulatory element

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN Viral / Regulación Viral de la Expresión Génica / Genoma Viral / Herpesvirus Suido 1 / G-Cuádruplex Tipo de estudio: Diagnostic_studies Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza

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