Novel tool to quantify cell wall porosity relates wall structure to cell growth and drug uptake.
J Cell Biol
; 218(4): 1408-1421, 2019 04 01.
Article
en En
| MEDLINE
| ID: mdl-30782779
Even though cell walls have essential functions for bacteria, fungi, and plants, tools to investigate their dynamic structure in living cells have been missing. Here, it is shown that changes in the intensity of the plasma membrane dye FM4-64 in response to extracellular quenchers depend on the nano-scale porosity of cell walls. The correlation of quenching efficiency and cell wall porosity is supported by tests on various cell types, application of differently sized quenchers, and comparison of results with confocal, electron, and atomic force microscopy images. The quenching assay was used to investigate how changes in cell wall porosity affect the capability for extension growth in the model plant Arabidopsis thaliana Results suggest that increased porosity is not a precondition but a result of cell extension, thereby providing new insight on the mechanism plant organ growth. Furthermore, it was shown that higher cell wall porosity can facilitate the action of antifungal drugs in Saccharomyces cerevisiae, presumably by facilitating uptake.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
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Pared Celular
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Arabidopsis
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Raíces de Plantas
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Epidermis de la Planta
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Aumento de la Célula
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Microscopía Fluorescente
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Antifúngicos
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
J Cell Biol
Año:
2019
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
Estados Unidos