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Atomic force microscopy-indentation demonstrates that alginate beads are mechanically stable under cell culture conditions.
Chui, Chih-Yao; Bonilla-Brunner, Andrea; Seifert, Jacob; Contera, Sonia; Ye, Hua.
Afiliación
  • Chui CY; Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, Old Road Campus off Roosevelt Drive, OX3 7DQ, United Kingdom.
  • Bonilla-Brunner A; Clarendon Laboratory, Department of Physics, University of Oxford, Parks Road, OX1 3PU, United Kingdom.
  • Seifert J; Clarendon Laboratory, Department of Physics, University of Oxford, Parks Road, OX1 3PU, United Kingdom; Department of Plant Sciences, University of Oxford, South Parks Road, OX1 3RB, United Kingdom.
  • Contera S; Clarendon Laboratory, Department of Physics, University of Oxford, Parks Road, OX1 3PU, United Kingdom. Electronic address: sonia.antoranzcontera@physics.ox.ac.uk.
  • Ye H; Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, Old Road Campus off Roosevelt Drive, OX3 7DQ, United Kingdom. Electronic address: hua.ye@eng.ox.ac.uk.
J Mech Behav Biomed Mater ; 93: 61-69, 2019 05.
Article en En | MEDLINE | ID: mdl-30772703
Alginate microbeads are extensively used in tissue engineering as microcarriers and cell encapsulation vessels. In this study, we used atomic force microscopy (AFM) based indentation using 20 µm colloidal probes to assess the local reduced elastic modulus (E * ) using a novel method to detect the contact point based on the principle of virtual work, to measure microbead mechanical stability under cell culture conditions for 2 weeks. The bead diameter and swelling were assessed in parallel. Alginate beads swelled up to 150% of their original diameter following addition of cell culture media. The diameter eventually stabilized from day 2 onwards. This behaviour was mirrored in E * where a significant decrease was observed at the start of the culture period before stabilization was observed at ~ 2.1 kPa. Furthermore, the mechanical properties of freeze dried alginate beads after re-swelling them in culture media were measured. These beads displayed vastly different structural and mechanical properties compared those that did not go through the freeze drying process, with around 125% swelling and a significantly higher E * at values over 3 kPa.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ensayo de Materiales / Microscopía de Fuerza Atómica / Técnicas de Cultivo de Célula / Alginatos / Fenómenos Mecánicos / Microesferas Idioma: En Revista: J Mech Behav Biomed Mater Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2019 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ensayo de Materiales / Microscopía de Fuerza Atómica / Técnicas de Cultivo de Célula / Alginatos / Fenómenos Mecánicos / Microesferas Idioma: En Revista: J Mech Behav Biomed Mater Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2019 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Países Bajos