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Ultrasound-enhanced scintillation proximity assay for rapid diagnostics.
Lee, So-Young; Lim, Jae-Cheong; Cho, Eun-Ha; Lee, Seung-Kon; Jung, Sung-Hee.
Afiliación
  • Lee SY; Radioisotope Research Division, Korea Atomic Energy Research Institute (KAERI), Daejeon, 305-353, Republic of Korea. Electronic address: solee31@kaeri.re.kr.
  • Lim JC; Radioisotope Research Division, Korea Atomic Energy Research Institute (KAERI), Daejeon, 305-353, Republic of Korea.
  • Cho EH; Radioisotope Research Division, Korea Atomic Energy Research Institute (KAERI), Daejeon, 305-353, Republic of Korea.
  • Lee SK; Radioisotope Research Division, Korea Atomic Energy Research Institute (KAERI), Daejeon, 305-353, Republic of Korea.
  • Jung SH; Radioisotope Research Division, Korea Atomic Energy Research Institute (KAERI), Daejeon, 305-353, Republic of Korea.
Anal Biochem ; 570: 51-55, 2019 04 01.
Article en En | MEDLINE | ID: mdl-30771337
Scintillation proximity assay (SPA) is a type of radioimmunoassay (RIA). We apply ultrasound enhancement to the general SPA. All assay procedures, including the antibody coating and radiolabeled antigen binding are achieved by simply mixing then standing for 5 min in an ultrasound chamber. No additional incubation time is required. To further demonstrate the capability of the UE-SPA, a quantitative measurement of CD55 in various grades of colon tumors was assessed on human tissue slides. The results showed a significant correlation between CD55 expression and tumorigenesis. In conclusion, we confirmed that UE-SPA is a reliable, rapid and alternative to RIA.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Radioinmunoensayo / Antígenos CD55 Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Anal Biochem Año: 2019 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Radioinmunoensayo / Antígenos CD55 Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Anal Biochem Año: 2019 Tipo del documento: Article Pais de publicación: Estados Unidos