Sufficient therapeutic effect of cryopreserved frozen adipose-derived regenerative cells on burn wounds.

Kaita, Yasuhiko; Tarui, Takehiko; Yoshino, Hideaki; Matsuda, Takeaki; Yamaguchi, Yoshihiro; Nakagawa, Takatoshi; Asahi, Michio; Ii, Masaaki

Kaita, Yasuhiko; Tarui, Takehiko; Yoshino, Hideaki; Matsuda, Takeaki; Yamaguchi, Yoshihiro; Nakagawa, Takatoshi; Asahi, Michio; Ii, Masaaki.

Afiliación

Kaita Y; Department of Trauma and Critical Care Medicine, Kyorin University School of Medicine, Tokyo, Japan.
Tarui T; Department of Trauma and Critical Care Medicine, Kyorin University School of Medicine, Tokyo, Japan.
Yoshino H; Division of Cardiology, Second Department of Internal Medicine, Kyorin University, School of Medicine, Tokyo, Japan.
Matsuda T; Department of Trauma and Critical Care Medicine, Kyorin University School of Medicine, Tokyo, Japan.
Yamaguchi Y; Department of Trauma and Critical Care Medicine, Kyorin University School of Medicine, Tokyo, Japan.
Nakagawa T; Department of Pharmacology, Faculty of Medicine, Osaka Medical College, Osaka, Japan.
Asahi M; Department of Pharmacology, Faculty of Medicine, Osaka Medical College, Osaka, Japan.
Ii M; Division of Research Animal Laboratory and Translational Medicine, Research and Development Center, Osaka Medical College, Osaka, Japan.

Regen Ther ; 10: 92-103, 2019 Jun.

Article en En | MEDLINE | ID: mdl-30766897

Palabras clave

ADRCs, adipose-derived regenerative cells; AdSCs, adipose-derived stem cells; Burn; CM, conditioned medium; DAPI, 4',6-diamidino-2-phenylindole; DMEM-F12, Dulbecco's modified Eagle's medium supplemented with F12; EGF, Epidermal growth factor; FBS, Fetal bovine serum; FGF2, Fibroblast growth factor 2; GAPDH, Glyceraldehyde 3-phosphate dehydrogenase; HGF, Hepatocyte growth factor; IGF-1, Insulin-like growth factor-1; ILB4, Isolectin B4; MSC, Mesenchymal stem cell; NHDF, Normal human dermal fibroblast; NHEK, Normal human epithelial keratinocyte; PBS, Phosphate-buffered saline; PVDF, Poly vinylidene di-fluoride; SDS-PAGE, Sodium dodecyl sulfate-polyacrylamide gel electrophoresis; SVF, Stromal vascular fraction; Stem cells; VEGF, Vascular endothelial growth factor; Wound healing; qRT-PCR, Quantitative real-time reverse transcription polymerase chain reaction

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Regen Ther Año: 2019 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Países Bajos

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