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Leaf Disc Assays for Rapid Measurement of Antioxidant Activity.
Kasote, Deepak M; Jayaprakasha, Guddadarangavvanahally K; Patil, Bhimanagouda S.
Afiliación
  • Kasote DM; Vegetable and Fruit Improvement Center, Department of Horticultural Sciences, Texas A&M University, 1500 Research Parkway, A120, College Station, TX, 77845-2119, USA.
  • Jayaprakasha GK; Vegetable and Fruit Improvement Center, Department of Horticultural Sciences, Texas A&M University, 1500 Research Parkway, A120, College Station, TX, 77845-2119, USA.
  • Patil BS; Vegetable and Fruit Improvement Center, Department of Horticultural Sciences, Texas A&M University, 1500 Research Parkway, A120, College Station, TX, 77845-2119, USA. b-patil@tamu.edu.
Sci Rep ; 9(1): 1884, 2019 02 13.
Article en En | MEDLINE | ID: mdl-30760761
Antioxidant levels are key parameters for studies of food quality, stress responses, and plant health. Herein, we have demonstrated that excised leaf disc has both radical scavenging activity and reducing power, and used this concept to develop 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and potassium permanganate reduction (PPR) leaf disc assays. Reaction time and reagent concentration for these assays were optimized using leaves from spinach, kale, collards, mustard, and watermelon. Further, these assays were validated for linearity and intra-assay precision. Ultra-high performance liquid chromatography coupled to an electrospray quadrupole time-of-flight mass spectrometer (UPLC/ESI-HR-QTOFMS) was used for phytochemical profiling and studying relative abundances of certain phenolic compounds in various leaf discs suspended and cell-free extracts. The mass spectral analysis showed that leaf disc suspended methanolic extracts had almost same phytochemical profiles to those of cell-free extracts. The DPPH leaf disc assay demonstrated better radical scavenging potential than the conventional cell-free extract method. By contrast, the observed antioxidant activity values in ABTS and PPR leaf disc assays were lower than those of conventional cell-free extract-based methods. In conclusion, the developed leaf disc assays are simple and rapid for the qualitative and comparative assessment of the antioxidant potential of leaf samples, as well as can be a good alternative to conventional cell-free extract based methods.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Brassica / Spinacia oleracea / Análisis por Micromatrices / Antioxidantes Tipo de estudio: Qualitative_research Idioma: En Revista: Sci Rep Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Brassica / Spinacia oleracea / Análisis por Micromatrices / Antioxidantes Tipo de estudio: Qualitative_research Idioma: En Revista: Sci Rep Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido