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Plant regeneration from hairy roots induced by infection with Agrobacterium rhizogenes in Crotalaria juncea L.
Ohara, A; Akasaka, Y; Daimon, H; Mii, M.
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  • Ohara A; Hokkaido National Agricultural Experiment Station, Shinsei, Memuro, Kasai, Hokkaido 0820071, Japan, , , , , , JP.
  • Akasaka Y; Faculty of Horticulture, Chiba University, Matsudo, Chiba 2718510, Japan e-mail: mii@midori.h.chiba-u.ac.jp Fax: +81-47-3088852, , , , , , JP.
  • Daimon H; College of Agriculture, Osaka Prefecture University, Sakai, Osaka 5998531, Japan, , , , , , JP.
  • Mii M; Faculty of Horticulture, Chiba University, Matsudo, Chiba 2718510, Japan e-mail: mii@midori.h.chiba-u.ac.jp Fax: +81-47-3088852, , , , , , JP.
Plant Cell Rep ; 19(6): 563-568, 2000 May.
Article en En | MEDLINE | ID: mdl-30754818
Hairy roots were induced from leaf segments of Crotalaria juncea, which is used as a green manure crop antagonistic to nematodes, by infection with a mikimopine type wild strain of Agrobacterium rhizogenes A13 (MAFF02-10266). These roots exhibited vigorous growth and abundant lateral branching on half-strength Murashige and Skoog (1/2MS) medium without phytohormones. The adventitious shoots were induced from 30% of root segments 3 months after transfer onto medium containing 3 mg/l benzyl adenine. These shoots produced roots 1 month after transfer onto 1.2% agar-solidified 1/2MS medium without phytohormones. Regenerated plants were successfully grown under greenhouse conditions. The transgenic nature of the regenerated plants was confirmed by Southern-blot analysis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Plant Cell Rep Asunto de la revista: BOTANICA Año: 2000 Tipo del documento: Article Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Plant Cell Rep Asunto de la revista: BOTANICA Año: 2000 Tipo del documento: Article Pais de publicación: Alemania