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Inhibition of fungal growth in planta and in vitro by transgenic tobacco expressing a bacterial nonheme chloroperoxidase gene.
Rajasekaran, K; Cary, J W; Jacks, T J; Stromberg, K D; Cleveland, T E.
Afiliación
  • Rajasekaran K; USDA, ARS, Southern Regional Research Center, 1100 Robert E. Lee Blvd., New Orleans, LA 70124, USA Fax: +1-504-2864217 e-mail: krajah@nola.srrc.usda.gov, , , , , , US.
  • Cary JW; USDA, ARS, Southern Regional Research Center, 1100 Robert E. Lee Blvd., New Orleans, LA 70124, USA Fax: +1-504-2864217 e-mail: krajah@nola.srrc.usda.gov, , , , , , US.
  • Jacks TJ; USDA, ARS, Southern Regional Research Center, 1100 Robert E. Lee Blvd., New Orleans, LA 70124, USA Fax: +1-504-2864217 e-mail: krajah@nola.srrc.usda.gov, , , , , , US.
  • Stromberg KD; Department of Biology, University of Southwestern Louisiana, Lafayette, LA 70504, USA, , , , , , US.
  • Cleveland TE; USDA, ARS, Southern Regional Research Center, 1100 Robert E. Lee Blvd., New Orleans, LA 70124, USA Fax: +1-504-2864217 e-mail: krajah@nola.srrc.usda.gov, , , , , , US.
Plant Cell Rep ; 19(4): 333-338, 2000 Mar.
Article en En | MEDLINE | ID: mdl-30754783
Transgenic tobacco plants producing chloroperoxidase (CPO-P), encoded by a novel gene from Pseudomonas pyrrocinia, were obtained by Agrobacterium-mediated transformation. Successful transformation was shown by PCR, Southern, northern and western blot analyses, and assays of CPO-P enzyme activity. Extracts from plants transformed with the CPO-P gene significantly reduced Aspergillus flavus colonies by up to 100% compared with extracts from control plants transformed with pBI121. Compared with controls, the transformed plants showed increased disease resistance in planta against a fungal pathogen, Colletotrichum destructivum, the causal agent of tobacco anthracnose.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Plant Cell Rep Asunto de la revista: BOTANICA Año: 2000 Tipo del documento: Article Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Plant Cell Rep Asunto de la revista: BOTANICA Año: 2000 Tipo del documento: Article Pais de publicación: Alemania