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Live Tracking of Inter-organ Communication by Endogenous Exosomes In Vivo.
Verweij, Frederik J; Revenu, Celine; Arras, Guillaume; Dingli, Florent; Loew, Damarys; Pegtel, D Michiel; Follain, Gautier; Allio, Guillaume; Goetz, Jacky G; Zimmermann, Pascale; Herbomel, Philippe; Del Bene, Filippo; Raposo, Graça; van Niel, Guillaume.
Afiliación
  • Verweij FJ; Institut Curie, PSL Research University, CNRS UMR144, Paris 75005, France; Institute for Psychiatry and Neuroscience Paris, Hopital Saint-Anne, Université Descartes, INSERM U894, Paris 75014, France. Electronic address: frederik.verweij@inserm.fr.
  • Revenu C; Institut Curie, PSL Research University, INSERM U934, CNRS UMR3215, Sorbonne Université, Paris 75005, France.
  • Arras G; Institut Curie, PSL Research University, Centre de Recherche, Laboratoire de Spectrométrie de Masse Protéomique, Paris, France.
  • Dingli F; Institut Curie, PSL Research University, Centre de Recherche, Laboratoire de Spectrométrie de Masse Protéomique, Paris, France.
  • Loew D; Institut Curie, PSL Research University, Centre de Recherche, Laboratoire de Spectrométrie de Masse Protéomique, Paris, France.
  • Pegtel DM; Department of Pathology, Cancer Center Amsterdam, the Netherlands.
  • Follain G; INSERM UMR_S1109, Université de Strasbourg, Fédération de Médecine Translationnelle de Strasbourg, Strasbourg, France.
  • Allio G; INSERM UMR_S1109, Université de Strasbourg, Fédération de Médecine Translationnelle de Strasbourg, Strasbourg, France.
  • Goetz JG; INSERM UMR_S1109, Université de Strasbourg, Fédération de Médecine Translationnelle de Strasbourg, Strasbourg, France.
  • Zimmermann P; Centre de Recherche en Cancérologie de Marseille, Aix-Marseille Université, Marseille 13284, France.
  • Herbomel P; Institut Pasteur, Department of Developmental & Stem Cell Biology, 25 rue du Dr Roux, Paris 75015, France.
  • Del Bene F; Institut Curie, PSL Research University, INSERM U934, CNRS UMR3215, Sorbonne Université, Paris 75005, France.
  • Raposo G; Institut Curie, PSL Research University, CNRS UMR144, Paris 75005, France.
  • van Niel G; Institut Curie, PSL Research University, CNRS UMR144, Paris 75005, France; Institute for Psychiatry and Neuroscience Paris, Hopital Saint-Anne, Université Descartes, INSERM U894, Paris 75014, France. Electronic address: guillaume.van-niel@inserm.fr.
Dev Cell ; 48(4): 573-589.e4, 2019 02 25.
Article en En | MEDLINE | ID: mdl-30745143
Extracellular vesicles (EVs) are released by most cell types but providing evidence for their physiological relevance remains challenging due to a lack of appropriate model organisms. Here, we developed an in vivo model to study EV function by expressing CD63-pHluorin in zebrafish embryos. A combination of imaging methods and proteomic analysis allowed us to study biogenesis, composition, transfer, uptake, and fate of individual endogenous EVs. We identified a subpopulation of EVs with exosome features, released in a syntenin-dependent manner from the yolk syncytial layer into the blood circulation. These exosomes are captured, endocytosed, and degraded by patrolling macrophages and endothelial cells in the caudal vein plexus (CVP) in a scavenger receptor- and dynamin-dependent manner. Interference with exosome biogenesis affected CVP growth, suggesting a role in trophic support. Altogether, our work represents a system for studying endogenous EV function in vivo with high spatiotemporal accuracy, demonstrating functional inter-organ communication by exosomes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transporte Biológico / Células Endoteliales / Exosomas / Vesículas Extracelulares Límite: Animals Idioma: En Revista: Dev Cell Asunto de la revista: EMBRIOLOGIA Año: 2019 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transporte Biológico / Células Endoteliales / Exosomas / Vesículas Extracelulares Límite: Animals Idioma: En Revista: Dev Cell Asunto de la revista: EMBRIOLOGIA Año: 2019 Tipo del documento: Article Pais de publicación: Estados Unidos