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The promoter of aBrassica napus polygalacturonase gene directs pollen expression ofß-glucuronidase in transgenicBrassica plants.
Hong, H P; Gerster, J L; Datla, R S S; Albani, D; Scoles, G; Keller, W; Robert, L S.
Afiliación
  • Hong HP; Plant Biotechnology Institute, National Research Council, 110 Gymnasium Road, S7N 0W9, Saskatoon, Saskatchewan, Canada.
  • Gerster JL; Eastern Cereal and Oilseed Research Centre, Central Experimental Farm, K1A 0C6, Ottawa, Ontario, Canada.
  • Datla RSS; Plant Biotechnology Institute, National Research Council, 110 Gymnasium Road, S7N 0W9, Saskatoon, Saskatchewan, Canada.
  • Albani D; Plant Biotechnology Institute, National Research Council, 110 Gymnasium Road, S7N 0W9, Saskatoon, Saskatchewan, Canada.
  • Scoles G; Department of Crop Science and Plant Ecology, University of Saskatchewan, S7N 5A8, Saskatoon, Saskatchewan, Canada.
  • Keller W; Plant Biotechnology Institute, National Research Council, 110 Gymnasium Road, S7N 0W9, Saskatoon, Saskatchewan, Canada.
  • Robert LS; Eastern Cereal and Oilseed Research Centre, Central Experimental Farm, K1A 0C6, Ottawa, Ontario, Canada.
Plant Cell Rep ; 16(6): 373-378, 1997 Mar.
Article en En | MEDLINE | ID: mdl-30727644
A 647-bp 5'-flanking fragment obtained from genomic clone Sta 44G(2) belonging to a family of polygalacturonase genes expressed inBrassica napus pollen was fused to theß-glucuronidase (GUS) marker gene. This fusion construct was introduced intoB. napus plants viaAgrobacterium tumefaciens transformation. Analysis of the transgenicB. napus plants revealed that this promoter fragment is sufficient to direct GUS expression specifically in the anther and that GUS activity increases in pollen during maturation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Plant Cell Rep Asunto de la revista: BOTANICA Año: 1997 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Plant Cell Rep Asunto de la revista: BOTANICA Año: 1997 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Alemania