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A mobile-phone based high-resolution microendoscope to image cervical precancer.
Grant, Benjamin D; Quang, Timothy; Possati-Resende, Júlio César; Scapulatempo-Neto, Cristovam; de Macedo Matsushita, Graziela; Mauad, Edmundo Carvalho; Stoler, Mark H; Castle, Philip E; Guerreiro Fregnani, José Humberto Tavares; Schmeler, Kathleen M; Richards-Kortum, Rebecca.
Afiliación
  • Grant BD; Department of Bioengineering, Rice University, Houston, Texas, United States of America.
  • Quang T; Department of Bioengineering, Rice University, Houston, Texas, United States of America.
  • Possati-Resende JC; Department of Cancer Prevention, Pio XII Foundation, Barretos Cancer Hospital, Barretos, Brazil.
  • Scapulatempo-Neto C; Molecular Oncology Research Center, Pio XII Foundation, Barretos Cancer Hospital, Barretos, Brazil.
  • de Macedo Matsushita G; Department of Pathology, Pio XII Foundation, Barretos Cancer Hospital, Barretos, Brazil.
  • Mauad EC; Department of Pathology, Pio XII Foundation, Barretos Cancer Hospital, Barretos, Brazil.
  • Stoler MH; Department of Cancer Prevention, Pio XII Foundation, Barretos Cancer Hospital, Barretos, Brazil.
  • Castle PE; Department of Pathology, University of Virginia School of Medicine, Charlottesville, VA, United States of America.
  • Guerreiro Fregnani JHT; Global Coalition against Cervical Cancer, Arlington, VA, United States of America.
  • Schmeler KM; Department of Epidemiology and Population Health, Albert Einstein College of Medicine, Bronx, NY, United States of America.
  • Richards-Kortum R; Center for the Researcher Support, Pio XII Foundation, Barretos Cancer Hospital, Barretos, Brazil.
PLoS One ; 14(2): e0211045, 2019.
Article en En | MEDLINE | ID: mdl-30726252
Nearly 90% of cervical cancer cases and deaths occur in low- and middle-income countries that lack comprehensive national HPV immunization and cervical cancer screening programs. In these settings, it is difficult to implement screening programs due to a lack of infrastructure and shortage of trained personnel. Screening programs based on visual inspection with acetic acid (VIA) have been successfully implemented in some low-resource settings. However, VIA has poor specificity and up to 90% of patients receiving treatment based on a positive VIA exam are over-treated. A number of studies have suggested that high-resolution cervical imaging to visualize nuclear morphology in vivo can improve specificity by better distinguishing precancerous and benign lesions. To enable high-resolution imaging in low-resource settings, we developed a portable, low-cost, high-resolution microendoscope that uses a mobile phone to detect and display images of cervical epithelium in vivo with subcellular resolution. The device was fabricated for less than $2,000 using commercially available optical components including filters, an LED and triplet lenses assembled in a 3D-printed opto-mechanical mount. We show that the mobile high-resolution microendoscope achieves similar resolution and signal-to-background ratio as previously reported high-resolution microendoscope systems using traditional cameras and computers to detect and display images. Finally, we demonstrate the ability of the mobile high-resolution microendoscope to image normal and precancerous squamous epithelium of the cervix in vivo in a gynecological referral clinic in Barretos, Brazil.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Displasia del Cuello del Útero / Neoplasias del Cuello Uterino / Colposcopía / Teléfono Celular / Microscopía Intravital Tipo de estudio: Diagnostic_studies / Screening_studies Límite: Female / Humans País/Región como asunto: America do sul / Brasil Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Displasia del Cuello del Útero / Neoplasias del Cuello Uterino / Colposcopía / Teléfono Celular / Microscopía Intravital Tipo de estudio: Diagnostic_studies / Screening_studies Límite: Female / Humans País/Región como asunto: America do sul / Brasil Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos