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Tendon Tissue Engineering: Effects of Mechanical and Biochemical Stimulation on Stem Cell Alignment on Cell-Laden Hydrogel Yarns.
Rinoldi, Chiara; Costantini, Marco; Kijenska-Gawronska, Ewa; Testa, Stefano; Fornetti, Ersilia; Heljak, Marcin; Cwiklinska, Monika; Buda, Robert; Baldi, Jacopo; Cannata, Stefano; Guzowski, Jan; Gargioli, Cesare; Khademhosseini, Ali; Swieszkowski, Wojciech.
Afiliación
  • Rinoldi C; Faculty of Material Science and Engineering, Warsaw University of Technology, Warsaw, 02-507, Poland.
  • Costantini M; Faculty of Material Science and Engineering, Warsaw University of Technology, Warsaw, 02-507, Poland.
  • Kijenska-Gawronska E; Institute of Physical Chemistry, Polish Academy of Sciences, Warsaw, 01-224, Poland.
  • Testa S; Faculty of Material Science and Engineering, Warsaw University of Technology, Warsaw, 02-507, Poland.
  • Fornetti E; Department of Biology, Tor Vergata Rome University, Rome, 00133, Italy.
  • Heljak M; Department of Biology, Tor Vergata Rome University, Rome, 00133, Italy.
  • Cwiklinska M; Faculty of Material Science and Engineering, Warsaw University of Technology, Warsaw, 02-507, Poland.
  • Buda R; Institute of Physical Chemistry, Polish Academy of Sciences, Warsaw, 01-224, Poland.
  • Baldi J; Institute of Physical Chemistry, Polish Academy of Sciences, Warsaw, 01-224, Poland.
  • Cannata S; Department of Orthopaedic Oncology, Regina Elena National Cancer Institute, Rome, 00100, Italy.
  • Guzowski J; Department of Applied Biotechnology and Translational Medicine, Tor Vergata Rome University, Rome, 00133, Italy.
  • Gargioli C; Department of Biology, Tor Vergata Rome University, Rome, 00133, Italy.
  • Khademhosseini A; Institute of Physical Chemistry, Polish Academy of Sciences, Warsaw, 01-224, Poland.
  • Swieszkowski W; Department of Biology, Tor Vergata Rome University, Rome, 00133, Italy.
Adv Healthc Mater ; 8(7): e1801218, 2019 04.
Article en En | MEDLINE | ID: mdl-30725521
Fiber-based approaches hold great promise for tendon tissue engineering enabling the possibility of manufacturing aligned hydrogel filaments that can guide collagen fiber orientation, thereby providing a biomimetic micro-environment for cell attachment, orientation, migration, and proliferation. In this study, a 3D system composed of cell-laden, highly aligned hydrogel yarns is designed and obtained via wet spinning in order to reproduce the morphology and structure of tendon fascicles. A bioink composed of alginate and gelatin methacryloyl (GelMA) is optimized for spinning and loaded with human bone morrow mesenchymal stem cells (hBM-MSCs). The produced scaffolds are subjected to mechanical stretching to recapitulate the strains occurring in native tendon tissue. Stem cell differentiation is promoted by addition of bone morphogenetic protein 12 (BMP-12) in the culture medium. The aligned orientation of the fibers combined with mechanical stimulation results in highly preferential longitudinal cell orientation and demonstrates enhanced collagen type I and III expression. Additionally, the combination of biochemical and mechanical stimulations promotes the expression of specific tenogenic markers, signatures of efficient cell differentiation towards tendon. The obtained results suggest that the proposed 3D cell-laden aligned system can be used for engineering of scaffolds for tendon regeneration.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estrés Mecánico / Tendones / Hidrogeles / Ingeniería de Tejidos / Andamios del Tejido Límite: Humans Idioma: En Revista: Adv Healthc Mater Año: 2019 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estrés Mecánico / Tendones / Hidrogeles / Ingeniería de Tejidos / Andamios del Tejido Límite: Humans Idioma: En Revista: Adv Healthc Mater Año: 2019 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Alemania