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Naked Liquid Marbles: A Robust Three-Dimensional Low-Volume Cell-Culturing System.
Chen, Mo; Shah, Megha P; Shelper, Todd B; Nazareth, Lynn; Barker, Matthew; Tello Velasquez, Johana; Ekberg, Jenny A K; Vial, Marie-Laure; St John, James A.
Afiliación
  • Chen M; Menzies Health Institute Queensland , Griffith University , Southport , 4222 Queensland , Australia.
  • Shah MP; Menzies Health Institute Queensland , Griffith University , Southport , 4222 Queensland , Australia.
  • Shelper TB; Menzies Health Institute Queensland , Griffith University , Southport , 4222 Queensland , Australia.
  • Nazareth L; Menzies Health Institute Queensland , Griffith University , Southport , 4222 Queensland , Australia.
  • Ekberg JAK; Menzies Health Institute Queensland , Griffith University , Southport , 4222 Queensland , Australia.
  • Vial ML; Menzies Health Institute Queensland , Griffith University , Southport , 4222 Queensland , Australia.
  • St John JA; Menzies Health Institute Queensland , Griffith University , Southport , 4222 Queensland , Australia.
ACS Appl Mater Interfaces ; 11(10): 9814-9823, 2019 Mar 13.
Article en En | MEDLINE | ID: mdl-30724549
Three-dimensional (3D) multicellular structures allow cells to behave and interact with each other in a manner that mimics the in vivo environment. In recent years, many 3D cell culture methods have been developed with the goal of producing the most in vivo-like structures possible. Whilst strongly preferable to  conventional cell culture, these approaches are often poorly reproducible, time-consuming, expensive, and labor-intensive and require specialized equipment. Here, we describe a novel 3D culture platform, which we have termed the naked liquid marble (NLM). Cells are cultured in a liquid drop (the NLM) in superhydrophobic-coated plates, which causes the cells to naturally form 3D structures. Inside the NLMs, cells are free to interact with each other, forming multiple 3D spheroids that are uniform in size and shape in less than 24 h. We showed that this system is highly reproducible, suitable for cell coculture, compound screening, and also compatible with laboratory automation systems. The low cost of production, small volume of each NLM, and production via automated liquid handling make this 3D cell-culturing system particularly suitable for high-throughput screening assays such as drug testing as well as numerous other cell-based research applications.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ensayos de Selección de Medicamentos Antitumorales / Esferoides Celulares / Técnicas de Cultivo de Célula / Ensayos Analíticos de Alto Rendimiento Límite: Humans Idioma: En Revista: ACS Appl Mater Interfaces Asunto de la revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Año: 2019 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ensayos de Selección de Medicamentos Antitumorales / Esferoides Celulares / Técnicas de Cultivo de Célula / Ensayos Analíticos de Alto Rendimiento Límite: Humans Idioma: En Revista: ACS Appl Mater Interfaces Asunto de la revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Año: 2019 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos