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Bacteriostatic and Bactericidal Activities of Camel Lactoferrins Against Salmonella enterica Serovar Typhi.
Almehdar, Hussein A; El-Baky, Nawal Abd; Alhaider, Abdulqader A; Almuhaideb, Saud A; Alhaider, Abdullah A; Albiheyri, Raed S; Uversky, Vladimir N; Redwan, Elrashdy M.
Afiliación
  • Almehdar HA; Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University, P.O. Box 80203, Jeddah, Saudi Arabia.
  • El-Baky NA; Therapeutic and Protective Proteins Laboratory, Protein Research Department, Genetic Engineering and Biotechnology Research Institute, City for Scientific Research and Technology Applications, New Borg EL-Arab, Alexandria, 21934, Egypt.
  • Alhaider AA; Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University, P.O. Box 80203, Jeddah, Saudi Arabia.
  • Almuhaideb SA; Tilad Veterinary Center, Riyadh, Saudi Arabia.
  • Alhaider AA; Medical school, King Saud bin Abdulaziz University for health Science, Riyadh, Saudi Arabia.
  • Albiheyri RS; Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University, P.O. Box 80203, Jeddah, Saudi Arabia.
  • Uversky VN; Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University, P.O. Box 80203, Jeddah, Saudi Arabia. vuversky@health.usf.edu.
  • Redwan EM; Institute for Biological Instrumentation of the Russian Academy of Sciences, Pushchino, Moscow region, 142290, Russia. vuversky@health.usf.edu.
Probiotics Antimicrob Proteins ; 12(1): 18-31, 2020 03.
Article en En | MEDLINE | ID: mdl-30723884
Lactoferrin is an iron-binding glycoprotein present in various secretions (e.g., milk, tears, saliva, pancreatic juice), which performs multiple functions, with one of them being the antimicrobial defense. Purified camel lactoferrins (cLfs) from different Saudi camel clans, as well as human and bovine lactoferrins (hLf or bLf) were tested as antimicrobial agents against Salmonella enterica serovar Typhi (S. Typhi). All cLfs showed superior antibacterial potentials relative to hLf or bLf, while there was no noticeable difference in the antimicrobial capabilities between the cLfs from different camel clans. We observed synergy between the inhibitory activities of Lfs and antibiotics against bacterial growth. Expression of numerous bacterial proteins was affected by the treatment with Lf and its combinations, giving insight into the molecular mechanisms of the Lf action. Furthermore, several bacterial proteins were shown to interact with cLf-biotin. Scanning and transmission electron microscopy revealed the presence of obvious extracellular and intracellular changes after S. Typhi treatment by antibiotic (carbenicillin) or cLf alone, and in combination. The effects of antibiotics and Lf were synergistic, supporting the potential of the use of Lf-antibiotic combinations.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Salmonella typhi / Leche / Lactoferrina / Antibacterianos Límite: Animals / Humans Idioma: En Revista: Probiotics Antimicrob Proteins Año: 2020 Tipo del documento: Article País de afiliación: Arabia Saudita Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Salmonella typhi / Leche / Lactoferrina / Antibacterianos Límite: Animals / Humans Idioma: En Revista: Probiotics Antimicrob Proteins Año: 2020 Tipo del documento: Article País de afiliación: Arabia Saudita Pais de publicación: Estados Unidos