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Molecular Classification and Comparative Taxonomics of Foveal and Peripheral Cells in Primate Retina.
Peng, Yi-Rong; Shekhar, Karthik; Yan, Wenjun; Herrmann, Dustin; Sappington, Anna; Bryman, Gregory S; van Zyl, Tavé; Do, Michael Tri H; Regev, Aviv; Sanes, Joshua R.
Afiliación
  • Peng YR; Center for Brain Science and Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.
  • Shekhar K; Klarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Yan W; Center for Brain Science and Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.
  • Herrmann D; Center for Brain Science and Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.
  • Sappington A; Klarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Bryman GS; F.M. Kirby Neurobiology Center and Department of Neurology, Boston Children's Hospital Harvard Medical School, Boston, MA 02115, USA.
  • van Zyl T; Department of Ophthalmology, Harvard Medical School, Boston, MA 02114, USA.
  • Do MTH; F.M. Kirby Neurobiology Center and Department of Neurology, Boston Children's Hospital Harvard Medical School, Boston, MA 02115, USA.
  • Regev A; Klarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Howard Hughes Medical Institute, Koch Institute of Integrative Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02140, USA.
  • Sanes JR; Center for Brain Science and Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA. Electronic address: sanesj@mcb.harvard.edu.
Cell ; 176(5): 1222-1237.e22, 2019 02 21.
Article en En | MEDLINE | ID: mdl-30712875
High-acuity vision in primates, including humans, is mediated by a small central retinal region called the fovea. As more accessible organisms lack a fovea, its specialized function and its dysfunction in ocular diseases remain poorly understood. We used 165,000 single-cell RNA-seq profiles to generate comprehensive cellular taxonomies of macaque fovea and peripheral retina. More than 80% of >60 cell types match between the two regions but exhibit substantial differences in proportions and gene expression, some of which we relate to functional differences. Comparison of macaque retinal types with those of mice reveals that interneuron types are tightly conserved. In contrast, projection neuron types and programs diverge, despite exhibiting conserved transcription factor codes. Key macaque types are conserved in humans, allowing mapping of cell-type and region-specific expression of >190 genes associated with 7 human retinal diseases. Our work provides a framework for comparative single-cell analysis across tissue regions and species.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Primates / Retina / Fóvea Central Límite: Aged / Animals / Female / Humans / Male Idioma: En Revista: Cell Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Primates / Retina / Fóvea Central Límite: Aged / Animals / Female / Humans / Male Idioma: En Revista: Cell Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos