S100 Proteins as Biomarkers in Risk Estimations for Malignant Transformation in Oral Lesions.

Probstmeier, Rainer; Kraus, Dominik; Wenghoefer, Matthias; Winter, Jochen

Probstmeier, Rainer; Kraus, Dominik; Wenghoefer, Matthias; Winter, Jochen.

Afiliación

Probstmeier R; Neuro- and Tumor Cell Biology Group, Department of Nuclear Medicine, University Hospital Bonn, University of Bonn, Bonn, Germany.
Kraus D; Department of Prosthodontics, Preclinical Education, and Material Sciences, University of Bonn, Bonn, Germany.
Wenghoefer M; Department of Oral and Maxillofacial Plastic Surgery, University of Bonn, Bonn, Germany.
Winter J; Department of Periodontology, Operative and Preventive Dentistry, University of Bonn, Bonn, Germany. jochen.winter@ukbonn.de.

Methods Mol Biol ; 1929: 763-771, 2019.

Article en En | MEDLINE | ID: mdl-30710310

RESUMEN

Oncologic relevant members of S100 proteins are described as promising biomarkers in molecular pathology for risk estimation in oral neoplasia exhibiting different stages of malignancy: gingiva as healthy tissue, irritation fibroma as benign, leukoplakia as precancerous, and oral squamous cell carcinoma as malignant entity. Gene expression levels of S100A4 (metastasin), S100A7 (psoriasin), S100A8 (calgranulin A), and S100A9 (calgranulin B) were analyzed using quantitative RT-PCR. In addition, immunohistochemistry-based microscopy was used to examine cellular localization and distribution of these biomarkers in tissue sections. The results indicate that S100 proteins represent promising biomarkers for early-stage diagnosis in oral lesions. The inclusion of expression profiles and ratios for each entity even improves their diagnostic validity.

Asunto(s)

Biomarcadores de Tumor/genética; Biomarcadores de Tumor/metabolismo; Transformación Celular Neoplásica/metabolismo; Neoplasias de la Boca/diagnóstico; Proteínas S100/genética; Proteínas S100/metabolismo; Calgranulina A/genética; Calgranulina A/metabolismo; Calgranulina B/genética; Calgranulina B/metabolismo; Transformación Celular Neoplásica/genética; Perfilación de la Expresión Génica; Regulación Neoplásica de la Expresión Génica; Humanos; Inmunohistoquímica; Neoplasias de la Boca/genética; Neoplasias de la Boca/metabolismo; Reacción en Cadena en Tiempo Real de la Polimerasa; Reacción en Cadena de la Polimerasa de Transcriptasa Inversa; Proteína A7 de Unión a Calcio de la Familia S100/genética; Proteína A7 de Unión a Calcio de la Familia S100/metabolismo; Proteína de Unión al Calcio S100A4/genética; Proteína de Unión al Calcio S100A4/metabolismo; Distribución Tisular

Palabras clave

Biomarkers; Calcium binding; EF-hand; Early-stage diagnosis; Molecular pathology; Oral lesions; S100 proteins

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Boca / Proteínas S100 / Biomarcadores de Tumor / Transformación Celular Neoplásica Tipo de estudio: Etiology_studies / Risk_factors_studies Límite: Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2019 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos

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