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Oxytocin facilitates the proliferation, migration and osteogenic differentiation of human periodontal stem cells in vitro.
Ge, Bin; Liu, Hongrui; Liang, Qianyu; Shang, Lingling; Wang, Ting; Ge, Shaohua.
Afiliación
  • Ge B; Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, Shandong Province, China; Department of Periodontology, School of Stomatology, Shandong University, Jinan, Shandong Province, China.
  • Liu H; Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, Shandong Province, China; Department of Periodontology, School of Stomatology, Shandong University, Jinan, Shandong Province, China.
  • Liang Q; Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, Shandong Province, China; Department of Periodontology, School of Stomatology, Shandong University, Jinan, Shandong Province, China.
  • Shang L; Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, Shandong Province, China; Department of Periodontology, School of Stomatology, Shandong University, Jinan, Shandong Province, China.
  • Wang T; Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, Shandong Province, China; Department of Periodontology, School of Stomatology, Shandong University, Jinan, Shandong Province, China.
  • Ge S; Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, Shandong Province, China; Department of Periodontology, School of Stomatology, Shandong University, Jinan, Shandong Province, China. Electronic address: shaohuage@sdu.edu.cn.
Arch Oral Biol ; 99: 126-133, 2019 Mar.
Article en En | MEDLINE | ID: mdl-30682715
OBJECTIVE: To explore the effect of oxytocin (OT) on the proliferation, migration, and osteogenic differentiation of periodontal ligament stem cells (PDLSCs) in vitro. DESIGN: PDLSCs were obtained by limiting dilution method. Immunofluorescence (IF), cell-counting kit-8 (CCK8), cell migration assay, Alizarin Red S staining, cetylpyridinium chloride (CPC) colorimetry, quantitative real-time polymerase chain reaction (qRT-PCR), and western blot analysis were used to examine the effect of OT on oxytocin receptor (OTR) expression, cell proliferation, migration and osteogenic differentiation of PDLSCs. RESULTS: Our study showed that PDLSCs expressed OTR. One hundred nanomolar OT exhibited the maximal effect on migration, while only 50 nM OT significantly promoted proliferation of PDLSCs, as well as mineralized nodule formation and calcium deposition (p < 0.05). Furthermore, 50 nM OT significantly up-regulated expression of osteogenesis-related genes-alkaline phosphatase (ALP), Collagen I (Col I), runt related transcription factor 2 (Runx 2), osteopontin (OPN) and osteocalcin (OCN)-at specific time points compared with osteogenic inductive medium (p < 0.05). In addition, western blot analysis demonstrated that 50 nM OT enhanced protein levels of ALP, Col I, and Runx 2 at day 7 and day 14 (p < 0.01), as well as activating the phosphorylation of extracellular-signal-regulated kinase (ERK) and protein kinase B (AKT) pathway; notably, 50 nM OT inhibited phosphorylation of the phosphatidylinositol 3-kinase (PI3K) pathway (p < 0.05). CONCLUSIONS: OT promoted proliferation, migration, and osteogenic differentiation of PDLSCs in vitro. Furthermore, the effect of OT on osteogenic differentiation was mediated through ERK and AKT pathway. Thus, OT may have potential for use in periodontal regeneration.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteogénesis / Ligamento Periodontal / Células Madre / Oxitocina / Diferenciación Celular / Movimiento Celular / Proliferación Celular Límite: Adolescent / Child / Humans Idioma: En Revista: Arch Oral Biol Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteogénesis / Ligamento Periodontal / Células Madre / Oxitocina / Diferenciación Celular / Movimiento Celular / Proliferación Celular Límite: Adolescent / Child / Humans Idioma: En Revista: Arch Oral Biol Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido