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Isolation and characterization of a bacteriophage and its potential to disrupt multi-drug resistant Pseudomonas aeruginosa biofilms.
Yuan, Yuyu; Qu, Kunli; Tan, Demeng; Li, Xiaoyu; Wang, Lili; Cong, Cong; Xiu, Zhilong; Xu, Yongping.
Afiliación
  • Yuan Y; School of Life Science and Biotechnology, Dalian University of Technology, Dalian, 116024, China.
  • Qu K; School of Life Science and Biotechnology, Dalian University of Technology, Dalian, 116024, China; BGI-Qingdao, 2877 Tuanjie Road, Sino-German Ecopark, Qingdao, 266000, China.
  • Tan D; Dalian SEM Bio-Engineering Technology Co. Ltd., Dalian 116620, China.
  • Li X; School of Life Science and Biotechnology, Dalian University of Technology, Dalian, 116024, China; Ministry of Education Center for Food Safety of Animal Origin, Dalian 116600, China.
  • Wang L; School of Life Science and Biotechnology, Dalian University of Technology, Dalian, 116024, China; Ministry of Education Center for Food Safety of Animal Origin, Dalian 116600, China.
  • Cong C; School of Life Science and Biotechnology, Dalian University of Technology, Dalian, 116024, China.
  • Xiu Z; School of Life Science and Biotechnology, Dalian University of Technology, Dalian, 116024, China.
  • Xu Y; School of Life Science and Biotechnology, Dalian University of Technology, Dalian, 116024, China; Ministry of Education Center for Food Safety of Animal Origin, Dalian 116600, China. Electronic address: xyping@dlut.edu.cn.
Microb Pathog ; 128: 329-336, 2019 Mar.
Article en En | MEDLINE | ID: mdl-30682523
A lytic Pseudomonas aeruginosa bacteriophage, vB_PaeM_LS1, was isolated and characterized herein. To examine the eligibility of bacteriophage vB_PaeM_LS1 as a therapeutic bacteriophage, we analysed its genome and compared it to similar bacteriophages. Genome of bacteriophage vB_PaeM_LS1 consisted of a linear, double-stranded DNA molecule 66,095 bp in length and with 55.7% G + C content. Neighbor-joining analysis of the large subunit terminase showed that bacteriophage vB_PaeM_LS1 had similarity to the Pbunavirus genus. The potential of the lytic bacteriophage to disrupt Pseudomonas aeruginosa biofilms was assessed by scanning electron microscopy and bacterial counts. This study revealed that the bacteriophage vB_PaeM_LS1 with its lytic effect showed a high potential impact on the inhibition of the growth of Pseudomonas aeruginosa biofilm formation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pseudomonas aeruginosa / Fagos Pseudomonas / Biopelículas Idioma: En Revista: Microb Pathog Asunto de la revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pseudomonas aeruginosa / Fagos Pseudomonas / Biopelículas Idioma: En Revista: Microb Pathog Asunto de la revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido