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VPAC1 receptors play a dominant role in PACAP-induced vasorelaxation in female mice.
Ivic, Ivan; Balasko, Marta; Fulop, Balazs D; Hashimoto, Hitoshi; Toth, Gabor; Tamas, Andrea; Juhasz, Tamas; Koller, Akos; Reglodi, Dora; Solymár, Margit.
Afiliación
  • Ivic I; Department of Anatomy, MTA-PTE PACAP Research Group, Medical School, University of Pecs, Pecs, Hungary.
  • Balasko M; Department of Physiology and Immunology, Medical School Osijek, University of J.J. Strossmayer, Osijek, Croatia.
  • Fulop BD; Institute for Translational Medicine, Medical School, University of Pecs, Pecs, Hungary.
  • Hashimoto H; Department of Anatomy, MTA-PTE PACAP Research Group, Medical School, University of Pecs, Pecs, Hungary.
  • Toth G; Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan.
  • Tamas A; Molecular Research Center for Children's Mental Development, United Graduate School of Child Development, Osaka University, Suita, Osaka, Japan.
  • Juhasz T; Division of Bioscience, Institute for Datability Science, Osaka University, Suita, Osaka, Japan.
  • Koller A; Department of Medical Chemistry, University of Szeged, Szeged, Hungary.
  • Reglodi D; Department of Anatomy, MTA-PTE PACAP Research Group, Medical School, University of Pecs, Pecs, Hungary.
  • Solymár M; Department of Anatomy, Histology, and Embryology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
PLoS One ; 14(1): e0211433, 2019.
Article en En | MEDLINE | ID: mdl-30682157
BACKGROUND: PACAP and VIP are closely related neuropeptides with wide distribution and potent effect in the vasculature. We previously reported vasomotor activity in peripheral vasculature of male wild type (WT) and PACAP-deficient (KO) mice. However, female vascular responses are still unexplored. We hypothesized that PACAP-like activity is maintained in female PACAP KO mice and the mechanism through which it is regulated differs from that of male PACAP KO animals. METHODS: We investigated the vasomotor effects of VIP and PACAP isoforms and their selective blockers in WT and PACAP KO female mice in carotid and femoral arteries. The expression and level of different PACAP receptors in the vessels were measured by RT-PCR and Western blot. RESULTS: In both carotid and femoral arteries of WT mice, PACAP1-38, PACAP1-27 or VIP induced relaxation, without pronounced differences between them. Reduced relaxation was recorded only in the carotid arteries of KO mice as compared to their WT controls. The specific VPAC1R antagonist completely blocked the PACAP/VIP-induced relaxation in both arteries of all mice, while PAC1R antagonist affected relaxation only in their femoral arteries. CONCLUSION: In female WT mice, VPAC1 receptors appear to play a dominant role in PACAP-induced vasorelaxation both in carotid and in femoral arteries. In the PACAP KO group PAC1R activation exerts vasorelaxation in the femoral arteries but in carotid arteries there is no significant effect of the activation of this receptor. In the background of this regional difference, decreased PAC1R and increased VPAC1R availability in the carotid arteries was found.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vasodilatación / Polipéptido Hipofisario Activador de la Adenilato-Ciclasa / Receptores de Tipo I del Polipéptido Intestinal Vasoactivo Límite: Animals Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2019 Tipo del documento: Article País de afiliación: Hungria Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vasodilatación / Polipéptido Hipofisario Activador de la Adenilato-Ciclasa / Receptores de Tipo I del Polipéptido Intestinal Vasoactivo Límite: Animals Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2019 Tipo del documento: Article País de afiliación: Hungria Pais de publicación: Estados Unidos