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Use of MALDI-TOF mass spectrometry to detect nosocomial outbreaks of Serratia marcescens and Citrobacter freundii.
Rödel, Jürgen; Mellmann, Alexander; Stein, Claudia; Alexi, Monika; Kipp, Frank; Edel, Birgit; Dawczynski, Kristin; Brandt, Christian; Seidel, Lothar; Pfister, Wolfgang; Löffler, Bettina; Straube, Eberhard.
Afiliación
  • Rödel J; Institute of Medical Microbiology, Jena University Hospital, Jena, Germany. juergen.roedel@med.uni-jena.de.
  • Mellmann A; Institute of Hygiene, University Hospital Münster, Münster, Germany.
  • Stein C; Institute of Infectious Diseases and Infection Control, Jena University Hospital, Jena, Germany.
  • Alexi M; Institute of Medical Microbiology, Jena University Hospital, Jena, Germany.
  • Kipp F; Institute of Infectious Diseases and Infection Control, Jena University Hospital, Jena, Germany.
  • Edel B; Institute of Medical Microbiology, Jena University Hospital, Jena, Germany.
  • Dawczynski K; Unit Neonatology, Department of Paediatrics, Jena University Hospital, Jena, Germany.
  • Brandt C; Institute of Infectious Diseases and Infection Control, Jena University Hospital, Jena, Germany.
  • Seidel L; bioMérieux, Nürtingen, Germany.
  • Pfister W; Institute of Medical Microbiology, Jena University Hospital, Jena, Germany.
  • Löffler B; Institute of Medical Microbiology, Jena University Hospital, Jena, Germany.
  • Straube E; Institute of Medical Microbiology, Jena University Hospital, Jena, Germany.
Eur J Clin Microbiol Infect Dis ; 38(3): 581-591, 2019 Mar.
Article en En | MEDLINE | ID: mdl-30680577
MALDI-TOF mass spectrometry (MS) may be used as a rapid typing method for nosocomial pathogens. Here, we evaluated MALDI-TOF MS for discrimination of hospital outbreak-related clusters of Serratia marcescens and carbapenemase-producing Citrobacter freundii. Thirty-three S. marcescens isolates collected from neonatal intensive care unit (NICU) patients, and 23 C. freundii isolates including VIM-positive isolates from a hospital colonization outbreak were measured by Vitek MS. Consensus spectra of each isolate were clustered using SARAMIS software. Genotyping was performed by whole-genome sequencing (WGS). First, a set of 21 S. marcescens isolates from 2014 with seven genotypes including three monoclonal clusters was used for the evaluation of MALDI-TOF typing. MS clustering was largely in agreement with genotyping results when the similarity cut-off for clonal identity was set on 90%. MALDI-TOF cluster analysis was then investigated for the surveillance of S. marcescens in the NICU in 2017 and demonstrated the introduction of new strains into the hospital and nosocomial transmissions. MS analysis of the C. freundii outbreak in 2016 revealed a monoclonal cluster of VIM-positive isolates and the separation of epidemiologically non-related VIM-positive and negative isolates. Two additional VIM-positive Citrobacter isolates from food samples were closely related to the large monoclonal cluster. WGS confirmed the MS results. MALDI-TOF MS may be used as a first-line typing tool for S. marcescens and C. freundii to detect transmission events in the hospital because isolates of an identical WGS type were grouped into the same MS cluster.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Serratia marcescens / Infección Hospitalaria / Técnicas de Tipificación Bacteriana / Citrobacter freundii / Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción / Infecciones por Enterobacteriaceae Tipo de estudio: Evaluation_studies Límite: Humans País/Región como asunto: Europa Idioma: En Revista: Eur J Clin Microbiol Infect Dis Asunto de la revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Serratia marcescens / Infección Hospitalaria / Técnicas de Tipificación Bacteriana / Citrobacter freundii / Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción / Infecciones por Enterobacteriaceae Tipo de estudio: Evaluation_studies Límite: Humans País/Región como asunto: Europa Idioma: En Revista: Eur J Clin Microbiol Infect Dis Asunto de la revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Alemania