Decellularized bovine placentome for portacavally-interposed heterotopic liver transplantation in rats.
Mater Sci Eng C Mater Biol Appl
; 97: 293-301, 2019 Apr.
Article
en En
| MEDLINE
| ID: mdl-30678914
Scaffolds from healthy placentae offer advantages for tissue engineering with undamaged matrix, associated cytoprotective molecules, and embedded vessels for revascularization. As size disparities in human placenta and small recipients hamper preclinical studies, we studied alternative of bovine placentomes in smaller size ranges. Multiple cow placentomes were decellularized and anatomical integrity was analyzed. Tissue engineering used inbred donor rat livers. Placentomes were hepatized and immediately transplanted in rats with perfusion from portal vein and drainage into inferior vena cava. Cows yielded 99⯱â¯16 placentomes each. Of these, approximately 25% had 3 to 9â¯cm diameter and 7 to 63â¯ml volume, which was suitable for transplantation. After decellularization, angiography and casts documented 100% of vessels and vascular networks were well-perfused without disruptions or leaks. The residual matrix also remained intact for transplantation of placentomes. Perfusion in transplanted placentomes was maintained over up to 30â¯days. Liver tissue reassembled with restoration of hepatic acinar and sinusoidal structure. Transplanted tissue was intact without apoptosis, or necrosis. Hepatic functions were maintained. Preservation of hepatic homeostasis was verified by cytofluorimetric analysis of hepatocyte ploidy. The prevalence in healthy and transplanted liver of diploid, tetraploid and higher ploidy classes was similar with 57%, 41% and 2% versus 51%, 46.5% and 2.6%, respectively, pâ¯=â¯0.77, ANOVA. CONCLUSIONS: Cow placentomes will allow therapeutic development with disease models in small animals. This will also advance drug or toxicology studies. Portasystemic interposition of engineered liver will be particularly suitable for treating hepatic insufficiencies (metabolic, secretory or detoxification needs), including for children or smaller adults.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Placenta
/
Trasplante de Hígado
/
Ingeniería de Tejidos
/
Hígado
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
/
Pregnancy
Idioma:
En
Revista:
Mater Sci Eng C Mater Biol Appl
Año:
2019
Tipo del documento:
Article
Pais de publicación:
Países Bajos