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The long noncoding RNA HOTTIP promotes breast cancer cell migration, invasiveness, and epithelial-mesenchymal transition via the Wnt-ß-catenin signaling pathway.
Han, Sijia; Jin, Xiaoming; Liu, Zhen; Xing, Fei; Han, Ye; Yu, Xiaopeng; He, Guijin; Qiu, Fang.
Afiliación
  • Han S; Department of Breast Surgery, Shengjing Hospital of China Medical University, Shenyang 110004, People's Republic of China.
  • Jin X; Department of Endocrinology, Northern Theater Command Airforce Hospital of Chinese PLA, Shenyang 110042, People's Republic of China.
  • Liu Z; Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang 110004, People's Republic of China.
  • Xing F; Department of Breast Surgery, Shengjing Hospital of China Medical University, Shenyang 110004, People's Republic of China.
  • Han Y; Department of Breast Surgery, Shengjing Hospital of China Medical University, Shenyang 110004, People's Republic of China.
  • Yu X; Department of Breast Surgery, Shengjing Hospital of China Medical University, Shenyang 110004, People's Republic of China.
  • He G; Department of Breast Surgery, Shengjing Hospital of China Medical University, Shenyang 110004, People's Republic of China.
  • Qiu F; Department of Breast Surgery, Shengjing Hospital of China Medical University, Shenyang 110004, People's Republic of China.
Biochem Cell Biol ; 97(5): 655-664, 2019 10.
Article en En | MEDLINE | ID: mdl-30676763
Long noncoding RNA HOTTIP (HOXA transcript at the distal tip) has recently been reported to have a role in the proliferation of various cancer cells, yet its role in cell migration, invasiveness, and the EMT (epithelial-mesenchymal transition) in breast cancer and the potential mechanisms remain unknown. Breast cancer cell lines MDA-MB-231 and MDA-MB-468 were transfected with shRNA (short hairpin RNA) that specifically targeting HOTTIP. We observed a remarkable decrease in migration and invasiveness in these two breast cancer cell lines after knock-down of HOTTIP by shHOTTIP. We also demonstrated that the EMT of these two breast cell lines was suppressed after HOTTIP knock-down, as evidenced by increased E-cadherin levels, and decreased levels of N-cadherin, Snail, and Twist. Moreover, HOTTIP silencing also suppressed tumor metastasis in nude mice in vivo. In addition, we found that the expression of ß-catenin was significantly decreased in breast cancer cells after knock-down of HOTTIP. In a further rescue experiment using overexpression of ß-catenin, the rates of cell migration, invasiveness, and EMT of HOTTIP-silenced breast cancer cells were promoted, disclosing a potential role of the Wnt-ß-catenin signaling pathway in this process. Overall, we discovered the positive regulatory function of HOTTIP in the migration, invasiveness, and EMT of breast cancer cells, via regulating the Wnt-ß-catenin pathway.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Mama / Movimiento Celular / Transición Epitelial-Mesenquimal / Vía de Señalización Wnt / ARN Largo no Codificante Límite: Animals / Female / Humans / Middle aged Idioma: En Revista: Biochem Cell Biol Asunto de la revista: BIOQUIMICA Año: 2019 Tipo del documento: Article Pais de publicación: Canadá
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Mama / Movimiento Celular / Transición Epitelial-Mesenquimal / Vía de Señalización Wnt / ARN Largo no Codificante Límite: Animals / Female / Humans / Middle aged Idioma: En Revista: Biochem Cell Biol Asunto de la revista: BIOQUIMICA Año: 2019 Tipo del documento: Article Pais de publicación: Canadá