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Mechanisms and immunogenicity of nsPEF-induced cell death in B16F10 melanoma tumors.
Rossi, Alessandra; Pakhomova, Olga N; Pakhomov, Andrei G; Weygandt, Samantha; Bulysheva, Anna A; Murray, Len E; Mollica, Peter A; Muratori, Claudia.
Afiliación
  • Rossi A; Old Dominion University, Frank Reidy Research Center for Bioelectrics, Norfolk, VA, 23508, USA.
  • Pakhomova ON; Old Dominion University, Frank Reidy Research Center for Bioelectrics, Norfolk, VA, 23508, USA.
  • Pakhomov AG; Old Dominion University, Frank Reidy Research Center for Bioelectrics, Norfolk, VA, 23508, USA.
  • Weygandt S; Old Dominion University, Frank Reidy Research Center for Bioelectrics, Norfolk, VA, 23508, USA.
  • Bulysheva AA; Old Dominion University, Frank Reidy Research Center for Bioelectrics, Norfolk, VA, 23508, USA.
  • Murray LE; SoBran Inc., 700 West Olney Road, Norfolk, VA, 23507, USA.
  • Mollica PA; Department of Medical Diagnostics and Translational Sciences, Old Dominion University, Norfolk, VA, 23508, USA.
  • Muratori C; Old Dominion University, Frank Reidy Research Center for Bioelectrics, Norfolk, VA, 23508, USA. cmurator@odu.edu.
Sci Rep ; 9(1): 431, 2019 01 23.
Article en En | MEDLINE | ID: mdl-30674926
Accumulating data indicates that some cancer treatments can restore anticancer immunosurveillance through the induction of tumor immunogenic cell death (ICD). Nanosecond pulsed electric fields (nsPEF) have been shown to efficiently ablate melanoma tumors. In this study we investigated the mechanisms and immunogenicity of nsPEF-induced cell death in B16F10 melanoma tumors. Our data show that in vitro nsPEF (20-200, 200-ns pulses, 7 kV/cm, 2 Hz) caused a rapid dose-dependent cell death which was not accompanied by caspase activation or PARP cleavage. The lack of nsPEF-induced apoptosis was confirmed in vivo in B16F10 tumors. NsPEF also failed to trigger ICD-linked responses such as necroptosis and autophagy. Our results point at necrosis as the primary mechanism of cell death induced by nsPEF in B16F10 cells. We finally compared the antitumor immunity in animals treated with nsPEF (750, 200-ns, 25 kV/cm, 2 Hz) with animals were tumors were surgically removed. Compared to the naïve group where all animals developed tumors, nsPEF and surgery protected 33% (6/18) and 28.6% (4/14) of the animals, respectively. Our data suggest that, under our experimental conditions, the local ablation by nsPEF restored but did not boost the natural antitumor immunity which stays dormant in the tumor-bearing host.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Melanoma Experimental / Terapia por Estimulación Eléctrica / Apoptosis Límite: Animals Idioma: En Revista: Sci Rep Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Melanoma Experimental / Terapia por Estimulación Eléctrica / Apoptosis Límite: Animals Idioma: En Revista: Sci Rep Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido