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Overexpression of ephrinB2 in stem cells from apical papilla accelerates angiogenesis.
Yuan, Changyong; Wang, Penglai; Zhu, Shaoyue; Liu, Zongxiang; Wang, Wen; Geng, Tengyu; Dissanayaka, Waruna Lakmal; Jin, Lijian; Zhang, Chengfei.
Afiliación
  • Yuan C; Endodontology, Faculty of Dentistry, The University of Hong Kong, Hong Kong, Hong Kong.
  • Wang P; HKU Shenzhen Institute of Research and Innovation, Shenzhen, China.
  • Zhu S; Dental Implant Center, Affiliated Xuzhou Stomatological Hospital of Xuzhou Medical University, Xuzhou, China.
  • Liu Z; Dental Implant Center, Affiliated Xuzhou Stomatological Hospital of Xuzhou Medical University, Xuzhou, China.
  • Wang W; Dental Implant Center, Affiliated Xuzhou Stomatological Hospital of Xuzhou Medical University, Xuzhou, China.
  • Geng T; Dental Implant Center, Affiliated Xuzhou Stomatological Hospital of Xuzhou Medical University, Xuzhou, China.
  • Dissanayaka WL; Dental Implant Center, Affiliated Xuzhou Stomatological Hospital of Xuzhou Medical University, Xuzhou, China.
  • Jin L; Dental Implant Center, Affiliated Xuzhou Stomatological Hospital of Xuzhou Medical University, Xuzhou, China.
  • Zhang C; Endodontology, Faculty of Dentistry, The University of Hong Kong, Hong Kong, Hong Kong.
Oral Dis ; 25(3): 848-859, 2019 Apr.
Article en En | MEDLINE | ID: mdl-30667136
OBJECTIVES: We aimed to accelerate angiogenesis in pulp regeneration by modulating ephrinB2 expression in stem cells from apical papilla (SCAPs). MATERIALS AND METHODS: Stem cells from apical papilla were transducted with ephrinB2-lentiviral expression vector (ephrinB2-SCAPs) in experimental group and green fluorescent protein (GFP-SCAPs) in control group. The transduction efficiency was confirmed by real-time PCR and Western blot assays. MTT assay was performed to detect the proliferative capacity of SCAPs after transduction. In vitro Matrigel assay and in vivo Matrigel plug assay were carried out to evaluate the angiogenic capacity. RESULTS: Results showed that ephrinB2-SCAPs had significantly higher ephrinB2 expression than GFP-SCAPs. EphrinB2-SCAPs upregulated vascular endothelial growth factor (VEGF) secretion under hypoxia. In vitro Matrigel assay demonstrated that human umbilical vein endothelial cells (HUVECs) cocultured with ephrinB2-SCAPs under hypoxia formed vascular-like structures earlier than GFP-SCAPs. Animal experiments confirmed that SCAPs co-transplanted with HUVECs enabled to generate greater amount of blood vessels than SCAPs alone. EphrinB2-SCAPs produced increased number of blood vessels with references to GFP-SCAPs, and those co-transplanted with HUVECs generated vessels with larger and functional tubule volumes. CONCLUSIONS: Regulating ephrinB2 expression in SCAPs may act as a new avenue for enhancing angiogenesis in dental pulp regeneration.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre / Neovascularización Fisiológica / Efrina-B2 / Pulpa Dental Límite: Animals / Female / Humans Idioma: En Revista: Oral Dis Asunto de la revista: ODONTOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Hong Kong Pais de publicación: Dinamarca
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre / Neovascularización Fisiológica / Efrina-B2 / Pulpa Dental Límite: Animals / Female / Humans Idioma: En Revista: Oral Dis Asunto de la revista: ODONTOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Hong Kong Pais de publicación: Dinamarca